姬松茸多糖对肾损伤小鼠血清中尿素氮和肌酐含量的影响

Effects of Agaicus Blazei Murrill Plysaccharid on Urea and Creatinine of Serum in Kidney Failure Mice

研究了姬松茸多糖对肾损伤小鼠血清中尿素氮和肝酐含量的影响。48只健康小鼠随机分为3组:健康对照组、病理模型组及多糖组。健康对照组不做任何处理;病理模型组及多糖组按0.15 mL/只,均腹腔注射庆大霉素,连续10 d,其中多糖组同时灌喂8%姬松茸多糖0.1 mL/只。在实验第10天,每只小鼠分别摘眼球取血并分离血清,测定小鼠血清中尿素氮和肌酐的含量。结果表明:病理模型组小鼠血清尿素氮和肌酐的含量与健康对照组相比,差异极显著(P<0.01),说明肾病理模型制造成功。而多糖组与病理模型组相比,小鼠血清尿素氮和肌酐的含量显著低于模型组(P<0.05)。本试验表明,连续10 d给小鼠腹腔注射0.15 mL的硫酸庆大霉素可以成功建立肾病理模型;而姬松茸多糖具有保护肾功能的作用。

The effects ofAgaicus blazei murrill plysaccharid on urea and creatinine of serum in kidney failure mice were tested in this study. 48 mice were randomly divided into three groups ： control group, pathological model group and polysaccharide group. The control group had no treatment and the other groups were given gentamicin by intraperitoneal injection respectively with doses of 0.15 mL for 10 days, while the polysaccharide group were given 8% polysaccharides with doses of 0,1 mL by oral administration. The levels of urea and creatinine in mice were detected. The results show that the level of serum urea and creatinine in the pathological group was significantly higher than the control group （P〈0.01） and the polysaccharide group was significantly lower than the pathological model group （P〈0.05）. Injecting 0.15 rnL gentamicin to tnice for 10 days can make ideal kidney failure models and Agaicus blazei murrill polysaccharide can enhance the protection for kidney.