结核性胸水中T淋巴细胞免疫表型分析

Analysis on immunophenotypes of T lymphocyte subsets in patients with tuberculosis

目的探讨结核性胸水中T淋巴细胞亚群的变化。方法采用流式细胞术检测30例结核患者胸水中CD45+CD3+CD4+、CD45+CD3+CD8+T淋巴细胞亚群。结果结核性胸水中淋巴细胞及CD4+T细胞比例显著升高,CD3+T细胞占有核细胞总数的(79.9±4.9)%;CD4+T细胞占总T细胞的(56.7±4.6)%;CD8+T细胞占总T细胞的(23.2±5.2)%。结论结核性胸水中存在特异性T淋巴细胞的集聚,CD4+、CD8+T细胞均参与结核的特异性免疫,其中CD4+T细胞起中心角色作用。

Objective To analyze the vatiations of T lymphocyte subsets of pleural effusion in patients with tuberculosis. Methods The CD45＋ CD3＋ CD4＋ , CD45＋ CD3＋ CD8＋ T lymphocyte subsets were detected by flow cytometry in the Pleural effusion in 30 tuberculosis patients. Results The patients with tuberculosis showed a significant increase in the percentage of T lymphocyte and CD4＋ cells of pleural effusion. There were （79.9±4.9）%of CD3＋ T cells in the total cells of Pleural effusion, （56.7±4.6）% of CD4＋ T cells and （23.2±5.2）% are CD8＋ T cells in the total T cells. Conclusion There were characteristic T cells groups in local pathologic samples of pleural effusion in tuberculosis patients. The data shows that both CD4＋ T cells and CD8＋ T cells have participated in specific cellular immunity of tuberculosis and CD4＋ T cells plays a major role.