6036例输血患者不规则抗体检测临床观察

Clinical observation of irregular antibodies detection of 6036 patients with blood transfusion

目的检测输血患者血清中不规则抗体,探讨不规则抗体筛查在临床输血中的意义。方法应用微柱凝胶免疫检测技术对本院2007年12月至2008年12月期间6 036例输血患者进行不规则抗体筛查,用盐水、酶、抗人球法进行抗体特异性鉴定。结果在6 036例输血患者中共筛查出抗体阳性13例,阳性率0.22%,其中自身抗体4例,确定同种特异性抗体9例,分别为抗-D 3例,抗-E 2例,抗-A11例,抗-Mur 1例,抗M 2例。结论输血前检测不规则抗体必不可少,特别对有输血史或妊娠史的患者更为重要,对保证患者输血安全、减少溶血性输血反应具有重要意义。

Objective To detect irregular antibody in the serum samples of patients with blood transfusion and explore the sig nificance of irregular antibody screening in clinical blood transfusion. Methods Microcolumn gel test was used for screening in 6 036 patients with blood transfusion during the period from December,2007 to December, 2008 and used saline,enzyme and anti- human globulin test for identificating antibody＇s specificity. Results There were 13 positive samples in 6036 patients with blood transfusion,and positive rate was 0.22%. Among them,4 samples were autoantibodies,and 9 samples were alloantibodies,including 3 anti-D,2 anti-E,1 anti-A1 , 1 anti Mur and 2 anti-M. Conclusion It is absolutely necessary in detecting irregular antibodies before blood transfusion,and more important for patients who had a history of blood transfusion or pregnancy specifically. There is impor rant significance in insuring the safety of the blood transfusion for patients,and reducing the hemolytic transfusion reaction.