摘要
经大量菌种初筛和复筛，得到一株产植酸酶较高的菌株Ｇ２，酶活达２．６千单位。菌种经ＵＶ和ＮＴＧ诱变处理，并采用高磷及磷霉素作筛子，得到了一株液体发酵产酶达１３．５千单位的菌株Ｇ２．１．１．５．７。
Founding and using micro plate method for determination of phytase activity.We got one strain Aspergillus niger (G2).G2 can produce 2 6Mu/ml phytase in shake flask fermentation.G2 was treated by UV and NTG, and high Pi and phosphomycin were used as inhibitors to screen high production strains.We got a mutant G2.1 1 5 7 It can produce 13 5Mu/ml phytase in shake flask fermentation.
出处
《西南农业学报》
CSCD
北大核心
1998年第4期18-22,共5页
Southwest China Journal of Agricultural Sciences
关键词
植酸酶
菌种筛选
菌种选育
饲料用酶
Phytase Micro organism screening Strains improving Micro plate method
