期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

小鼠HMGN2干扰质粒psilence-cmv-4.1-HMGN2构建及体内干扰效果鉴定 被引量：2

Construction and identification of mouse shRNA psilencer-cmv-4.1-HMGN2
下载PDF
导出
摘要 目的:构建小鼠HMGN2干扰质粒psilence-cmv-4.1-HMGN2,以进一步明确HMGN2的生物学作用。方法:将HMGN2-shRNA片段退火后与小鼠psilence-cmv-4.1链接,饲养孕小鼠,尾静脉注射法导入shRNA表达质粒,提取8.5d胚胎RNA,用RT-PCR检测胎HMGN2的表达量进行鉴定。结果:测序结果显示psilence-cmv-4.1-HMGN2干扰质粒构建成功,RT-PCR结果显示HMGN2在8.5d胎儿表达较高广泛表达,且Psilencer-cmv-4.1-HMGN2尾静脉注射后有明显的抑制效果。结论:成功构建对8.5d胎鼠HMGN2基因具有显著干扰效率的psilencer-4.1-HMGN2干扰质粒,为进一步研究HMGN2基因的功能打下了基础。 Objective： To construct highly efficient mouse shRNA psilencer-c mv-4. 1-HMGN2, and the transcription mechanism of HMGN2. Methods. shRNA psileneer-c mv-4. 1-HMGN2 was constructed and was transmitted into the pregnant ICR mice with tail venous injection. At 8. 5 days in gestation, embryo RNA was extracted and identified the expression of HMGN2 by RT-PCIL Resuits：The shRNA psilenee-emv-4. 1-HMGN2 was constructed successfully and the expression of HMGN2 was higher at 8. 5 days in gestation embryo, and significantly downregulated by shRNA psilencer-c mv-4. 1-HMGN2. Conclusion. The psilenee-c mv-4. 1- HMGN2 was constructed successfully and the interference efficiency was 70%.
作者 韩琴 邓璐霞 赵静 吴桂霞 曹玥 范波 李夏 陈善泽 高祥 黄宁
机构地区 四川大学华西医学中心感染与免疫实验室 成都医学院公共卫生实验中心
出处 《四川生理科学杂志》 2010年第1期5-8,共4页 Sichuan Journal of Physiological Sciences
基金 国家自然科学基金(30671963) CMB基金(98-683)
关键词 HMGN2 Psilencer-cmv-4.1-HMGN2 HMGN2 Psilencer-c mv-4. 1-HMGN2
分类号 R346 [医药卫生—基础医学]
  • 相关文献

参考文献12

  • 1Landsman D, Soares N, Gonzalez FJ, et al. Chromosomal protein HMG-17. Complete human cDNA sequence and evidence for a multigene family[J]. J Biol Chem, 1986, 261(16): 7479-7484.
  • 2Srikantha T, Landsman D, Bustin M. Retropseudogenes for human chromosomal protein HMG-17[J]. J Mol Biol, 1987, 197(3): 405 -413.
  • 3Landsman D,McBride OW, Bustin M. Human non-histone chromosomal protein HMG-17 : identification, characterization, chromosome localization and RFLPs of a functional gene from the large multigene family[J]. Nucleic Acids Res, 1989, 17(6): 2301-2314.
  • 4Strichman-Almashanu LZ, Bustin M, Landsman D. Retroposed copies of the HMG genes: a window to genome dynamics[J]. Genome Res, 2003, 13(5): 800-812.
  • 5Kazmierczak B, Dal Cin P, Rogalla P, et al. Regional fine mapping of HMG17 to chromosomal band 1p35[J]. Cancer Genet Cytogenet, 2000, 116(2): 164-165.
  • 6Sparrow DB, Wells JR. Sequence of a cDNA encoding chicken highmobility-group protein-2[J]. Gene, 1992, 114(2): 289-290.
  • 7Tremethiek DJ, Hyman L High mobility group protein 14 and 17 can prevent the close packing of nucleosomes by increasing the strength of protein contacts in the linker DNA[J]. J Biol Chem, 1996, 271(20): 12009-12016.
  • 8Swerdlow PS,Varshavsky A. Affinity of HMG17 for a mononucleosome is not influenced by the presence of ubiquitin-H2A semihistone but. strongly depends on DNA fragment size. Nucleie Acids Res. 1983 Jan 25 ; 11(2) :387-401.
  • 9Spieker N, Beitsma M, Van Sluis P, et al. An integrated 5-Mb physical,genetic,and radiation hybrid map of a 1p36. 1 region implicated in neuroblastoma pathogenesis [J ]. Genes Chromosomes Cancer, 2000, 27(2): 143-152.
  • 10Tzioufas AG, Boumba VA, Seferiadis K, et al. Autoantibodies to HMG-17 nucleosomal protein in autoimmune rheumatic diseases. Correlation with systemic lupus erythematosus clinical activity and with antibodies to double-stranded DNA [J]. Arthritis Rheum, 1993, 36(7): 955-961.

同被引文献14

  • 1Bustin M. Regulation of DNA-dependent activities by the functional motifs of the high-mobility-group chromosomal proteins[J].Molecular and Cellular Biology,1999,(08):5237-5246.
  • 2Hock R,Scheer U,Bustin M. Chromosomal proteins HMG-14 and HMG-17 are released from mitotic chromosomes and imported into the nucleus by active transport[J].Journal of Cell Biology,1998,(06):1427-1436.
  • 3Spieker N,Beitsma M,van Sluis P. An integrated 5-Mb physical,genetic,and radiation hybrid map of a lp36.1 region implicated in neuroblastoma pathogenesis[J].Genes,Chromosomes and Cancer,2000,(02):143-152.
  • 4Feng Y,He F,Zhang P. Inhibitory effect of HMGN2 protein on human hepatitis B virus expression and replication in the HepG2.2.15 cell line[J].Antiviral Research,2009,(03):277-282.
  • 5Feng Y,Huang N,Wu Q. HMGN2:A novel antimicrobial effector molecule of human mononuclear leukocytes[J].Journal of Leukocyte Biology,2005,(05):1136-1141.
  • 6Bustin M,Reeves R. High-mobility-group chromosomal proteins:Architectural components that facilitate chromatin function[J].Progress in Nucleic Acid Research and Molecular Biology,1996.35-100.
  • 7Bustin M,Lehn DA,Landsman D. Structural features of the HMG chromosomal proteins and their genes[J].Biochimica Et Biophysica Acta,1990,(03):231-243.
  • 8Ding HF,Bustin M,Hansen U. Alleviation of histone H1-mediated transcriptional repression and chromatin compaction by the acidic activation region in chromosomal protein HMG-14[J].Molecular and Cellular Biology,1997,(10):5843-5855.
  • 9Trieschmann L,Postnikov YV,Rickers A. Modular structure of chromosomal proteins HMG-14 and HMG-17:Definition of a transcriptional enhancement domain distinct from the nucleosomal binding domain[J].Molecular and Cellular Biology,1995,(12):6663-6669.
  • 10Porkka K,Laakkonen P,Hoffman JA. A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo[J].Proceedings of the National Academy of Sciences(USA),2002,(11):7444-7449.doi:10.1073/pnas.062189599.

引证文献2

二级引证文献3

四川生理科学杂志
2010年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部