大鼠肺动脉平滑肌细胞钾通道的电生理学特性

Study on the properties of potassium channels of pulmonary artery smooth muscle cells isolated from rats

目的:研究大鼠肺动脉平滑肌细胞钾通道的电生理学特性。方法:用急性酶分离法分离单个大鼠肺动脉平滑肌细胞,采用全细胞膜片钳技术对肺动脉平滑肌细胞钾通道的电生理学特性进行研究。结果:在一定的实验条件下可记录出钙激活性钾电流(Kca)和电压门控性钾电流(Kv)。Kca可被四乙胺阻断,Kv电流由快速失活K+电流(Ka)、缓慢失活钾电流(Kst)和延迟整流K+电流(KDR)以不同比例复合而成,可被四氨基吡啶所阻断。结论:利用膜片钳技术研究发现,低氧通过作用K+通道引起肺动脉平滑肌细胞收缩。

Objective： To The properties of the potassium channels of pulmonary artery smooth muscle cells isolated from rats were studied. Methods. The patch-clamp techniques in the conventional whole-cell configuration were used. Results. The results indicated that voltage-gated potassium currents and calcium-activated K^＋ currents can be recorded under given conditions. Calcium-activated K^＋ currents can be inhibited by 5mmol L^-1 TEA, voltage-gated potassium currents, which can be inhibited by 5 mmol L^-1 4-AP, contained at least three components： a rapidly inactivating potassium current （Ka）, a slowly inactivating potassium eurrant （Kst） and delayed rectifier potassium current （KDR）. In different ceils these three components constituted voltage-gated potassium current in different proportion, Conclusions-We can learn the properties of the potassium channels of pulmonary artery smooth muscle cells with the： patch-clamp techniques.