醒脑静注射液对内毒素休克家兔血浆细胞因子含量的影响

Effect of Xingnaojing injection (XNJ) on levels of tumor necrosis factor-α and interleukin-10 in rabbits of endotoxin shock

目的:探讨醒脑静注射液治疗内毒素休克的可能机制。方法:日本大耳白兔18只,随机分为正常对照组、LPS组、LPS+XNJ组,每组各6只。采用耳缘静脉注射LPS复制兔内毒素休克模型,分别进行治疗,于治疗后0、1、2、3h观察各组平均动脉压(MAP)、心率(HR)、存活率,并取血用ELISA双抗体夹心法检测血浆TNF-α及IL-10的含量。结果:LPS组平均动脉压在0h后迅速下降,心率明显加快,2h后又迅速减慢;PLS+XNJ组平均动脉压在0h后平缓下降,心率先加快后平缓减慢。LPS组存活率为0,PLS+XNJ组存活率为100%。TNF-α的变化:LPS组TNF-α0h开始增高,3h达到顶峰;LPS+XNJ组TNF-α在0h达到顶峰,1h明显下降,3h降至最低。IL-10的变化:LPS组IL-10在3h达到顶峰,PLS+XNJ组在2h达到顶峰,3h开始下降。结论:醒脑静注射液能治疗内毒素休克,其机制可能与调节促炎因子和抗炎因子的平衡有关。

Objective： To investigate the effect of Xingnaojing injection （XNJ） on endotoxin shock in rabbits and study the possible mechanism. Methods： 18 healthy male rabbits were divided into 3 groups randomly： （1）normal group （n=6） ; （2）LPS group （n=6） ; （3）PLS＋XNJ group （n=6）. Each group was established model with LPS, and treated with XNJ injection respectively. Watched the MAP. BP. survival rate, and take blood sample to measure the level of TNF-α and IL-10. Results： In LPS group the MAP declined rapidly at Oh. The HR accelerated obviously at Oh and decreased rapidly at 2h. In LPS＋XNJ group the MAP declined gently at Oh. The HR accelerated firstly and declined gently. The survival rate of LPS group was 0 and LPS＋XNJ group was 100%. The change of TNF-α： TNF-α raised at Oh and achieved peak at 3h in LPS group. In LPS＋XNJ group TNF-α raised and achieved peak at Oh, then went down at lh obviously, reach lowest at 3h. The change of IL-10：IL-10 achieved peak at 3h in LPS group. In LPS＋XNJ group IL-10 achieved peak at 2h, then went down at 3h. Conclusion： Xingnaojing injection can improve the endotoxin shock, and the possible mechanism is relative to adjust the balance of pro-inflammatory factor and anti-inflammatory factor.