MTHFR基因C677T和A1298C基因多态性与乳腺癌的相关性的初步探讨

SINGLE NUCLEOTIDE POLYMORPHISMS IN METHYLENETETRAHYDROFOLATE REDUCTASE GENE AND SUSCEPTIBILITY TO BREAST CANCER

目的探讨代谢叶酸的亚甲基四氢叶酸还原酶(MTHFR)基因单核苷酸多态与乳腺癌风险的关系。方法以聚合酶链反应和限制性片段长度多态方法,分析了65个乳腺癌病人和143个正常对照的MTHFR基因C677T和A1298C基因型及其对乳腺癌风险的相关性。结果MTHFRC677T和A1298C基因型在病例和对照中的分布差异无显著性意义。1298变异基因型与677变异基因型之间无有协同作用。结论MTHFRC677T和A1298C单核苷酸多态可能与乳腺癌的遗传易感性无关。

Objective To explore the relationship between genetic polymorphisms in methylenetetrahydrofolate reductase （MTHFR） ,a central enzyme in folate metabolism that affects DNA methylation and synthesis, and the risk of Breast Cancer. Methods Genomic DNA was isolated from the peripheral lymphocytes of 65 patients with Breast Cancer and 143 control subjects. Polymcrise chain reaction and restriction fragment length polymorphism were used to examine the polymorphisms of MTHFR genes C6677T and A1298C and the relation between these genotypes and risk of Breast Cancer was analyzed. Results The distribution of MTHFR C677T and A1298C genotype was not sinnificantly different between the two groups. Conclusion Sinnle nucleotide polymorphism in the MTHFR gene is not a risk factor of Breast Cancer. thus showing that no a joint effect exists between the MTHFR677 and 1298 polymorphisms on the risk of Breast Cancer.