治疗型关节炎护膝对兔膝骨关节炎软骨细胞增殖和凋亡的影响

Influence of OA Knee Pad on Cartilage Cell Proliferation and Apoptosis of Rabbit with Knee Osteoarthritis

目的:探讨OA护膝对兔膝骨性关节炎软骨细胞增殖、凋亡的影响。方法:6月龄兔54只,采用改良Hulth法复制膝骨性关节炎模型,随机分为6组,正常组常规饲养,模型组造模后常规饲养,对照组微波仪治疗,实验1组电(疏密波)治疗,实验2组热(热软膜),实验3组电热(OA护膝),治疗30 min,1次/d,连续治疗16周时处死,采用免疫组化检测软骨细胞增殖、凋亡。结果:16周时,关节软骨细胞凋亡率,各试验组明显高于正常组(P<0.01,P<0.05),对照组、实验1组和实验2组、实验3组明显低于模型组(P<0.01,P<0.05),实验3组明显低于对照组、实验1组和实验2组(P<0.05);关节软骨细胞增殖率,各试验组明显低于正常组(P<0.01,P<0.05),对照组、实验1组和实验2组、实验3组明显高于模型组(P<0.01,P<0.05),实验3组高于对照组、实验1组和实验2组(P<0.05)。结论:OA护膝能有效促进关节软骨细胞增殖,抑制软骨细胞凋亡。

Objective：To investigate the influence of the OA knee pad on cartilage cell proliferation and apoptosis of rabbits with knee osteoarthritis.Methods：54 rabbits of 6-months-old copied knee osteoarthritis by modified Hulth Law were randomly divided into 6 groups.Normal group received the conventional breeding,model group received conventional breeding after modeled,and control group received the microwave therapy.Experimental group 1 received the electricity（density wave） in the treatment,experimental group 2 received the hot（hot soft membrane） in the treatment and experimental group 3 received electrothermal（OA knee pad） in the treatment,30 minutes each once,once a day.All of them were killed after the treatment for 16 weeks.The cartilage cell proliferation and apoptosis was detected by immunohistochemical method.Results：After 16 weeks,the cartilage cell apoptosis rate was significantly higher in test group than normal group（P〈0.01,P〈0.05）,control group,experimental group 1,2 and 3 was significantly lower than model group（P〈0.01,P〈0.05）,experimental group 3 was significantly lower than control group,experimental group 1 and 2（P〈0.05）.Articular cartilage cell proliferation rate,the test group was significantly lower than normal group（P〈0.01,P〈0.05）,control group,experimental group 1,2 and 3 was significantly higher than model group（P〈0.01,P〈0.05）,experimental group 3 was significantly higher than control group,experimental group 1 and 2（P〈0.05）.Conclusion：OA knee articular cartilage can be effective in promoting cell proliferation,inhibit chondrocyte apoptosis.