HPLC测定妇乐颗粒中川楝素含量

Quantitative Determination of Toosendanin in Fule Keli by HPLC

目的:建立测定妇乐颗粒中川楝素含量的HPLC方法。方法:色谱柱为Agilent zorbax SB-C18柱(4.6mm×150mm,5μm),流动相为乙腈-水(30:70),流速为1.0mL·min-1,检测波长为210nm。结果:川楝素在0.5～5μg·mL-1范围内呈良好的线性关系(r=0.9999),平均回收率为99.31%,RSD为0.73%(n=9)。结论:该方法快速、简便、准确,可用于本制剂的质量控制。

Objective： To establishes a HPLC method for the quantitative determination of toosendanin in Fule Keli. Methods： HPLC was performed on Agilest zorbax SB-C18 （ 4.6mm × 150mm, 5 μm ）with column at room temperature. The mobile phase consisted of aeetonitrile-water （ 30 ： 70 ） at a flow rate and detection wavelength were set at 1.0mL · min^-1 and 210nm. Results： The linear range of toosendanin was 0.5-5 μg· mL^-1 （r=0.9999）, the mean recovery was99.31% with RSD 0.73% （n=9）. Conclusion： The method is fast, simple and accurate, and it can be applied for the quality control of Fule Keli.