黄芪多糖对暴露于苯、甲醛环境中小鼠脾脏的保护作用

Protective Effects of Astragalus Polysaccharides on Spleen Tissues from Mice Exposed to Compound of Benzene and Formaldehyde

目的探讨黄芪多糖对暴露于苯、甲醛环境中小鼠脾脏的保护作用。方法将40只雄性小鼠随机分成4组,即对照组、苯甲醛染毒组(苯:5 g/m3,甲醛:50 mg/m3)、苯甲醛染毒黄芪多糖低剂量保护组[黄芪多糖:10 mg/(kg.d),苯:5 g/m3,甲醛:50 mg/m3]、苯甲醛染毒黄芪多糖高剂量保护组[黄芪多糖:20 mg/(kg.d),苯:5 g/m3,甲醛:50 mg/m3]。采用静式吸入染毒,每天2 h,连续30 d,并同时采用饮水的方式给予黄芪多糖保护。末次染毒24 h内,处死小鼠,对小鼠脾中谷胱甘肽(GSH)含量及谷胱甘肽过氧化物酶(GSH-PX)活性进行测定和分析。结果苯、甲醛染毒黄芪多糖保护各组脾组织中GSH含量和GSH-PX活性均高于苯、甲醛染毒组,均有统计学意义(P<0.01)而与对照组的差异无统计学意义(P>0.05),苯、甲醛染毒黄芪多糖保护组高剂量组脾组织中GSH-PX活性高于低剂量组,差异有统计学意义(P<0.01)。结论黄芪多糖对暴露于苯、甲醛环境中小鼠脾脏具有保护作用,且对脾中GSH含量的作用有随剂量增加而增大的趋势,对脾中GSH-PX活性的作用随剂量增加而增大。

Objective To study the protective effect of Astragalus polysaccharides on spleen tissues.Methods Forty male mice were divided into 4 groups randomly（10 in each group）： control group,benzene and formaldehyde group with no polysaccharide protection（benzene： 5 g/m^3,formaldehyde： 50 mg/m^3）,low dose of Astragalus polysaccharides group with benzene and formaldehyde [Astragalus polysaccharides： 10 mg/（kg·d）,benzene： 5 g/m^3,formaldehyde： 50 mg/m^3],high dose of Astragalus polysaccharides group with benzene and formaldehyde [Astragalus polysaccharides： 20 mg/（kg·d）,benzene： 5 g/m^3,formaldehyde： 50 mg/m^3].The mice were exposed to benzene and formaldehyde 2 hours per day for 30 days continuously,and offered water with Astragalus polysaccharides,the mice were killed within 24 hours after the last exposure.The content of glutathione（GSH） and the activities of glutathione peroxidase（GSH-PX） were determined and detected in spleen tissues.Results The contents of GSH and the activities of GSH-PX in spleen tissues in mice from the Astragalus polysaccharides protecting groups were significantly higher than that of the benzene and formaldehyde group（P〈0.01）,and had no significant difference to the control groups（P〈0.05）.The activities of GSH-PX in spleen tissues of the low dose Astragalus polysaccharides protecting group were significantly lower than that of the high dose Astragalus polysaccharides protecting group（P〈0.01）.Conclusions／ It is suggested that Astragalus polysaccharides could protect the spleen tissues from benzene and formaldehyde and better protection with higher dose of Astragalus polysaccharides.