体外皮肤刺激模型的生物标志研究进展

Progress on Biomarkers of Skin Irritation Models in vitro

皮肤刺激试验是人类健康相关产品危险性评价的常见项目,传统皮肤刺激试验采用实验动物进行,成本高周期长,给动物造成一定程度的痛苦。近年来,多种替代动物试验的体外模型被开发和应用。体外试验主要通过定量检测细胞活性和代谢变化的生物标志物预测体内的效应,应用最广泛的生物标志物是细胞活性、炎性因子、胞质酶等,在生物技术的推动下,新的特异性标志物被开发和验证。

Skin irritant test was the routinely conducted for human healthy related product hazard assessment.Traditional skin irritancy testing used lab animals,costly,time-consuming and suffering from stress.Recent years,diverse alternative models for skin irritancy have been developed and applied.The effects in vivo were predicted in vitro testing by quantitative measured cell viability and biomarkers for metabolism disturbing.The biomarkers for skin irritancy included viability,inflammation cytokines,enzymes,morphology,etc.New specially biomarkers will be developed and validated under biotechnique improvement.