丙型肝炎病毒准种血清学检测技术的建立

The Serology Detection for Hepatitis C Virus Quasispecies

目的:建立一种以血清学为基础的丙型肝炎病毒(HCV)准种检测技术。方法:自20份HCV血清中各挑选30个克隆进行测序比较,分析HCV准种的复杂程度;以HCV准种代表性抗原组合制备免疫芯片,用血清学检测技术分析上述20份HCV血清中的准种变异程度;比较两种方法之间的检出灵敏度和相关性。结果:测序法检出灵敏度为70.0%,血清学检测法检出灵敏度为95.0%,后者显著高于前者(P<0.05);两种方法检测结果的相关性为74.7%(P<0.01)。结论:血清学检测技术操作简单,且能够反映丙型肝炎患者的HCV准种变异程度,适于临床推广。

Objective：To establish the technology for hypervarible region 1（HVR1） of hepatitis C virus（HCV） by serology detection and compare its results to clone sequencing.Methods：RT-PCR sequence analysis and serology detection were used to find out the variation of HVR1,then their relativity and sensitivity were compared.Results：The sensitivity of the sequence analysis was 70.0%,compared to 95.0% in the serology detection,which was more sensitive obviously（P0.05）.The relativity between the two methods was 75%（P0.01）.Conclusion：The serology detection for HVR1 is consistency with clone sequencing and it can reflect the HVR1 variation in the HCV positive patients.The technology is suitable for clinical treat direction.