氯化甲基汞抗裸鼠皮下U251胶质瘤细胞的体内实验研究

The morphology study in apoptosis effect of methyl-mercuric chloride on brain neuroglioma

目的在建立裸鼠皮下U251胶质瘤模型基础上,通过体内实验及相关形态学研究,分析氯化甲基汞(MMC)的抗脑胶质瘤作用。方法制备裸鼠皮下U251胶质瘤模型,随机分为对照组和实验组,实验组裸鼠注射U251脑胶质瘤细胞1w后,每天灌胃给予10mg/kg体重MMC,连续5d,对照组给予相同体积的生理盐水。全部裸鼠于肿瘤接种后14d处死。结果大体病理显示实验组肿瘤体积明显小于对照组,病理组织学观察发现实验组U251胶质瘤细胞生长密度较对照组低,并可见细胞体积变小、核固缩等凋亡的形态学改变。透射电镜显示实验组肿瘤细胞发生核固缩、染色质周边化、核断裂、胞浆空泡变性等改变,实验组瘤体汞含量明显高于对照组。结论MMC对裸鼠U251胶质瘤细胞增殖具有明显的抑制作用,其机制可能是通过诱导胶质瘤细胞凋亡实现的,相关分子机制有待进一步深入研究。

Objective To study the morphology change in apoptosis effect of methyl-mercuric chloride （MMC） on brain neuroglioma.Methods Experimental group were lavaged with MMC for 5 d and 10 mg/kg BW per day after the conduction of the athymic rat model of neuroglioma,control group was given with sodium chloride at the same dose.Results Oncopathology study showed that the average tumor volume of experimental group was smaller than those in control group obviously,the experimental group showed a lower density and a smaller volume than those in control group from HE stain.The phenomenon of karyopycnosis,liquefaction and anachromasis also could be seen in some cells.The neuroglioma cells were represented pyknosis,chromoplasm margination,nuclear fragmentation and vacuolar degeneration by perspective electron microscope.Conclusions MMC is capable of inhibiting the proliferation of rat C6 neuroglioma cells in vivo.The possible mechanism is that MMC is capable of deducing apoptosis in neuroglioma cells.The molecule mechanism of inhibitory effect of MMC on brain neuroglioma are requested for research to advance.