rhIGF-1对成骨细胞增殖、BMP-2及Cbfa1基因表达的影响

Effect of recombinant human insulin-like growth factor-1(rhIGF-1) on the proliferation,bone morphogenetic protein-2(BMP-2) and core binding factor a1(Cbfa1)gene expression in cultured rat osteoblast

目的通过重组人胰岛素样生长因子(rhIGF-1)对体外成骨细胞增殖、骨形态蛋白-2(BMP-2)及核心结合因子1(Cbfa1)基因表达的影响,探讨rhIGF-1对骨代谢影响的可能机制。方法不同浓度的rhIGF-1(0、10、50、100ng/ml)刺激体外培养的大鼠成骨细胞,采用噻唑蓝(MTT)法检测细胞增殖能力,用半定量RT-PCR法检测成骨细胞BMP-2、Cbfa1基因的表达。结果rhIGF-1可明显促进成骨细胞增殖(P<0.05),并促进成骨细胞BMP-2、Cbfa1基因的表达(P<0.01),具有浓度依赖性。结论rhIGF-1可促进成骨细胞的增殖、分化及成熟,可能是通过增强BMP-2、Cbfa1基因的表达来实现的。

Objective To study the effect of rhIGF-1 on the proliferation,BMP-2 and Cbfal gene expression in rat osteoblast in vitro. Methods Cultured rat osteoblast were treated with rhIGF-1 in different concentrations（0, 10,50,100 ng/ml）. Cell proliferation was assessed by MTT colorimetric assay, the expression of BMP-2, Cbfal gene in rat osteoblast were examined by semiquantitive RT-PCR. Results rhIGF-1 can promote osteoblast proliferation （P 〈 0. 05） and increase the expression of BMP-2 and Cbfal gene in a does-dependent manner （P 〈 0.01 ）. Conclusions rhIGF-1 induces the proliferation, differentiation and maturity of osteoblast probably by increasing the expression of BMP-2 and Cbfal gene.