甲亢患者血清和尿液的核磁共振代谢组学研究

NMR-based Metabonomics Study on Serum and Urine of Hyperthyroidism

应用基于核磁共振(NMR)的代谢组学方法,研究甲状腺功能亢进(简称甲亢)患者和健康人群的血清和尿液,分析甲亢疾病的特征代谢物.实验收集33个甲亢患者和17个健康志愿者的血清样品以及53个甲亢患者和58个健康志愿者的尿液样品,采用多元统计分析方法研究甲亢组和对照组血清和尿液中的内源性代谢差异.结果表明,甲亢组血清中的胆碱、葡萄糖和三甲胺等物质的含量升高,而VLDL,LDL和胆固醇等脂质以及乳酸、糖蛋白和丙氨酸等代谢物的含量下降;甲亢组尿液中的葡萄糖、柠檬酸、牛磺酸以及肌氨酸等代谢物的含量升高,而马尿酸、TMAO、甲酸和琥珀酸等代谢物的含量下降.结果表明,甲亢病不仅影响了糖类、脂类和蛋白质三大物质的代谢,还对能量代谢、肝肠循环和肠道微生物等多个生理系统产生显著影响,并且可能造成肝脏及肾脏等器官的损伤.

Nuclear magnetic resonance（NMR） based metabonomics was applied to study the hyperthyroidism by analyzing metabolic profiling of serum and urine.The purpose of this study is to determine an array of characteristic metabolites in serum and urine samples from hyperthyroidism patients and then to interpret these metabolites in possible metabolic pathway.Serum samples from 33 hyperthyroidism patients together with 17 healthy volunteers and urine samples from 53 hyperthyroidism patients with 58 healthy volunteers were collec-ted.Differences in endogenous metabolites were detected on serum and urine samples from the hyperthyroidism group and control group using multivariate statistical analysis.The results show that the hyperthyroidism group has elevated levels of choline,glucose and declined levels of VLDL,LDL,cholesterol,lactate,glycoprotein and alanine in serum samples relative to the healthy group.In urine samples,the hyperthyroidism group show increased levels of glucose,citrate,taurine and creatinine as well as decreased levels of hippurate,trimethylamine-N-oxide,formate and succinate.These results indicate that hyperthyroidism have not only disturbed the carbohydrate metabolism,lipid metabolism and protein metabolism but also influenced energy metabolism,hepatoenteral circulation and gut microflora and caused liver and kidney injury.