巨噬细胞移动抑制因子-173G/C基因多态性与紫癜性肾炎的相关性

Correlation between-173 G/C Gene Polymorphism of Macrophage Migration Inhibitory Factor and Henoch-Schonlein Purpura Nephritis

目的探讨江西地区汉族儿童巨噬细胞移动抑制因子(MIF)-173G/C基因多态性与过敏性紫癜(HSP)、紫癜性肾炎(HSPN)的相关性。方法HSP患儿131例[并肾炎80例(HSPN组),未并肾炎51例(单纯HSP组)]与健康对照儿童105例,均于晨起空腹采集外周静脉血2 mL,应用Promega人全血基因组DNA试剂盒提取其外周血基因组DNA;PCR法扩增目的DNA片段,内切酶(AluⅠ)酶切消化产物,采用限制性片段长度多态性(RFLP)方法分析MIF-173位点多态性,凝胶电泳图像系统判断基因型。采用频率计数法计算单纯HSP组、HSPN组和健康对照组各基因型及等位基因频率。采用SPSS11.5软件对各基因型及等位基因频率差异进行χ2检验,计算比值比(OR)及95%可信区间(95%CI),以估计各基因型对疾病发生的相对危险度。结果MIF-173G/C位点检测出3种基因型,分别为GG型、GC型、CC型。单纯HSP组和健康对照组仅检出GG和GC型,而HSPN组3种基因型均检出。HSPN组MIF-173基因突变型(37.5%)和C等位基因频率(20.0%)均高于健康对照组(分别为20.0%、10.0%)(χ2=6.964、7.400,Pa<0.01)。结论MIF-173G/C基因多态性与儿童HSPN的发生有关,C等位基因可能是儿童HSPN的易感基因。

Objective To explore the correlation between - 173G/C gene polymorphism of macrophage migration inhibitory, factor （MIF） and Henoch -Schonlein purpnra（HSP） , Henoch -Schonlein purpura nephritis（HSPN） in children in Jiangxi Province. Methods One hundred and thirty - one ethnic Han children with HSP were enrolled, including 80 children with concurrent nephritis （HSPN group） and 51 children without nephritis （ HSP without nephritis group）. One hundred and five healthy children were used as the healthy control group. Germline DNA was extracted from peripheral blood by Promega blood genomic DNA kit. Polymerase chain reaction - restriction fragment length poly- morphism（PCR- RFLP） was used for genotyping the -173G/C polymorphism of MIF. Genotype distribution and allele frequencies were obtained by direct counting. Statistical analysis was performed by using SPSS 11.5 software. Allele and genotype distribution were compared by using the chi -square test. The relative risk of allele was described by odds ratios （OR） and 95 % confidence intervals （95 % CI）. Results Three genotypes（ GG, GC, CC ） were detected in MIF - 173 G/C. GG, GC genotypes were detected in HSP without nephritis and healthy control group. GG, GC and CC genotypes were detected in HSPN group. Mutant genotype （37.5%） and C allele frequency （20.0%） in HSPN group were significantly higher than those in healthy control group （20.0% and 10.0%, respectively） （X^2 = 6. 964,7. 400, P 〈 0. 01 ）. Conclusions MIF - 173G/C gene Dolymorohism mac be associated with the develooment of HSPN.C allele may be a susceptible gene of HSPN.