摘要
目的:研究乙型肝炎病毒(HBV)感染者外周血单个核细胞(PBMCs)中HBVDNA的存在及其与血清HBVDNA的关系。方法:用PCR法及斑点杂交法对50例HBsAg(+)的感染者PBMCsHBVDNA进行检测。同时用PCR法检测感染者血清HBVDNA。结果:PCR法和斑点杂交法分别从35份和32份PBMCs检出HBVDNA,阳性率分别为70.0%和64.0%,各种临床型乙型肝炎PBMCsHBVDNA检出率均为60.0%左右。PCR法检测血清HBVDNA阳性率为36.0%。结论:HBV存在于HBV感染者PBMCs中,并且HBV可单独存在于感染者PBM-Cs。
Objective:This paper was to study the presence of HBV DNA in mononuclear cells from peripheral blood (PBMCs) among subjects infected with hepatitis B virus and its relationship with serum HBV DNA. Methods:PBMCs and serum samples were examined by PCR and Dot blot hybridization in 50 patients with HBsAg(+) in serum. Results:By PCR,HBV DNA was detected in PBMCs from 35 of 50 patients(70%). By dot blot hybridization, we found HBV DNA in 32 (64.0%). With respect to different types of clinical Hepatitis B, the positive rate of HBV DNA in PBMCs was 60.0%. The positive rate of HBV DNA in serum was 36.0%. Conclusions:HBV DNA could exist in PBMCs independently. We found no obvious difference in the positive rate of HBV DNA from PBMCs among all kinds of clinical hepatitis B.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1998年第6期566-570,共5页
Journal of China Medical University