口腔鳞癌组织中蛋白激酶C及其抑制物活性的研究

Protein Kinase C and Its Inhibitor Activity in Oral Squamous Cell Carcinomas

目的：探讨口腔鳞癌发生的分子生物学机理。方法：采用Ｔａｋａｉ法研究２０例口腔鳞癌及其临近正常组织的蛋白激酶Ｃ（ＰｒｏｔｅｉｎｋｉｎａｓｅＣ，ＰＫＣ）及其抑制物（ＰｒｏｔｅｉｎｋｉｎａｓｅＣｉｎｈｉｂｉｔｏｒ，ＰＫＣＩ）活性。结果：与临近正常组织相比口腔鳞癌胞浆ＰＫＣ活性明显升高（Ｐ＜０．０１），而胞膜无明显变化（Ｐ＞０．０５）；与临近正常组织相比口腔鳞癌胞浆ＰＫＣＩ活性明显降低（Ｐ＜０．０１），而胞膜无明显变化（Ｐ＞０．０５）。结论：口腔鳞癌的发生与ＰＫＣ及ＰＫＣＩ在亚细胞水平活性变化密切相关。

Objective: The paper was to explore the mocular mechanism of canceration in oral squamous cell carcinomas. Methods: We used Takai's method to study activity of protein kinase C (PKC) and its inhibitor (PKCI) in oral squamous cell carcinomas and their adjacent normal tissues from twenty patients. Results: The activity of PKC increased significantly (P<0.01) and that of PKCI decreased obviously (P<0.01) in cancerous cytoplasm as compared with that in adjacent tissues. No apparent change occured in cancerous cell membrane for the activity of PKC and PKCI in comparison with that in normal cells (P>0.05). Conclusions: The activity of PKC and PKCI was closely related to the canceration of the oral squamous cell carcinomas.