摘要
[目的]克隆水稻ISA1基因,分析ISA1基因在不同组织和不同灌浆期胚乳中的表达情况。[方法]以粳稻品种日本晴为试验材料,采用半定量RT-PCR技术分析ISA1基因在不同组织和不同灌浆期胚乳中的表达情况。[结果]水稻淀粉去分支酶1cDNA序列ORF编码811个氨基酸残基;同源性比较和系统进化树分析表明,水稻ISA1基因与其他物种已发表的ISA1基因核苷酸序列和推导的氨基酸序列的同源性分别在66.8%～83.0%和69.0%～83.3%,且与大麦、小麦等物种亲缘关系最近;半定量RT-PCR分析表明,ISA1基因在叶、根、茎中不表达,在灌浆期12d胚乳中的表达量最大。[结论]该研究结果为进一步研究ISA1基因的表达和调控机制奠定了基础。
[Objective] The aim was to clone ISA1 from Oryza sativa and analyze its expression situation in different tissues and different endosperm filling stage. [Method] With japonica rice cultivar nipponbare as test material,the expression situation of ISA1 in different tissues and different endosperm filling stage was analyzed by using semi-quantitative RT-PCR technique. [Result]The full length open reading fragments of ISA1 encoded 811 amino acid residues. The homologous alignment and phylogenetic analysis showed that the similarities of nucleotide acid sequences and deduced amino acid sequences of ISA1 of rice to those of some other plants were 66.8%-83.0% and 69.0%-83.3%,respectively,and had a relatively high degree of similarity with barley and wheat. The semi-quantitative RT-PCR analysis indicated that ISA1 expressed with a peak value at 12 d after pollination in endosperm but not in root,stem or leaf. [Conclusion] The study laid a foundation for further study on the expression and regulatory mechanism of ISA1 gene.
出处
《安徽农业科学》
CAS
北大核心
2010年第9期4440-4441,4444,共3页
Journal of Anhui Agricultural Sciences
基金
浙江省自然科学基金项目(Y3090617
Y304463)
