小麦对白粉菌过敏性反应与H_2O_2及3种氧化酶活性变化的关系

Relationship between the Hypersensitive Response of Wheat to Blumeria graminis f. sp. tritici and Hydrogen Peroxide,Three Enzyme Activities Changes

[目的]研究小麦对白粉菌过敏性反应与H2O2及3种氧化酶活性变化的关系,为探讨小麦对白粉菌抗性生理机制奠定基础。[方法]以白粉菌菌株17(Bt17)和菌株6(Bt6)及小麦品种扬158为试验材料,测定H2O2处理的小麦叶片过敏性细胞数量及POD、PPO、SOD酶活性。[结果]乳突在亲和性与非亲和性组合叶表细胞形成的差异不大,非亲和性反应叶表细胞过敏性反应发生早且明显比亲和性反应多;接种Bgt17后不同时间及非亲和性组合中含H2O2的过敏性反应细胞频率明显高于亲和性组合;用5、10、20mmol/L抗坏血酸处理后,小麦叶片中过敏性细胞数量显著减少,15.0与30.0mmol/LH2O2处理后诱导亲和性反应小麦叶片中过敏性细胞数量显著增加;接种白粉菌后,小麦叶麦中POD、PPO、SOD活性均呈先上升后下降趋势,且非亲合组合比亲合组合3种酶的活性高。[结论]H2O2、PPO、SOD活性与小麦对白粉菌抗性存在一定相关性。

[Objective] The research aimed to study the relationship between the hypersensitive response of wheat to Blumeria graminis f.sp.tritici and hydrogen peroxide,three enzyme activities changes and lay the foundation for discussing the resistant physiological mechanism of wheat to B. graminis. [Method] Taking B. graminis Bt 17 and Bt 6 and wheat cultivar Yang 158 as test materials,the number of hypersensitive cells and activities of POD,PPO and SOD in wheat leaves treated by H2O2 were determined. [Result] The mastoid had little difference between compatibility and incompatibility leaf surface cells. The hypersensitive response of incompatibility leaf surface cells occurred early and it was more than compatibility reaction obviously. The frequency of hypersensitive reaction with H2O2 in incompatibility combinations after inoculating Bgt17 for different time was obviously higher than compatibility combinations. After the leaves were treated by 5,10,20 mmol/L ascorbic acid,the number of hypersensitive cells in wheat leaves decreased dramatically. The number of hypersensitive cells in wheat leaves inducing the compatibility after treatment with 15.0 and 30.0 mmol/L H2O2 significantly increased. After inoculating B. graminis,the activities of POD,PPO and SOD showed a trend of first increasing and then decreasing. And the activities of three enzymes in incompatibility combinations were higher than compatibility combinations. [Conclusion] The activities of POD,PPO and SOD had certain correlation with the resistance of wheat to B. graminis.