犬弓首线虫卵及幼虫排泄分泌抗原对小鼠的免疫保护作用

IMMUNE PROTECTION OF LARVAE ES ANTIGENS AND ULTRAVIOLET IRRADIATED EMBRYONATED EGGS OF TOXOCARA CANIS ON MICE

目的：探索犬弓首线虫幼虫移行症免疫预防的可能性。方法：采用经紫外线照射的犬弓首线虫感染期卵管喂和犬弓首线虫第二期幼虫排泄分泌抗原（TES-Ag）皮下注射免疫小鼠，末次免疫后2周，用犬弓首线虫感染期虫卵管喂攻击感染，1000个卵／鼠。攻击感染后1周解剖鼠，用稀盐酸分离法分离幼虫计数。结果：紫外线照射虫卵免疫组和TES-Ag200μg免疫组的减虫率分别为27．5％和53．7％。结论：紫外线照射虫卵和TES-Ag免疫鼠均产生了一定的保护免疫力。

Objective: To explore the possibility of immune prevention of larva migrans of Toxocara canis. Methods: KM mice were drimunized orally with ultraviolet irrwhated embryonated eggs, or subcutaneously with Toxocara canis second boae ES antigens to investigate the immune protechon against T.canis infection. One week ther the final inununization, sera were collected from mice to measure antibody with double diffusion test. Two weeks after the final immunisation, mice were challenged with 1000 infechve eggs of T. canis. The mice were dissected and larvae isolated and counted a week later. Result:Specific antibody was induced and the reducing rate of larva in ThS-Ag 200μg immune group and in irradiated embryonated eare drimune group was 53. 7%, and 27. 5% respectively. Conclusion: Both lareae ES antigens and ultraviolet irradiated eggs might cfford proptection against T. canis infection.