石见穿总酚酸对小鼠四氯化碳急性肝损伤的保护作用

Hepatoprotective Effects of Phenolic Acid from Salvia chinensis Benth. on Carbon Tetrachloride-Induced Acute Liver Injury in Mice

[目的]研究石见穿总酚酸对受四氯化碳损伤的小鼠肝脏保护作用及其可能机制。[方法]将60只昆明种小鼠随机分为对照组和模型组,石见穿设高、中、低剂量组,阳性对照组(水飞蓟宾),共6个组。灌胃给药连续5d,皮下注射1ml/kg50%四氯化碳花生油溶液,24h测定血清天门冬氨基酸转移酶(AST)、丙氨酸氨基转移酶(ALT)及组织中总抗氧化能力(T-AOC)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量。[结果]与模型组比较,石见穿总酚酸高、中、低剂量组血清AST、ALT活性均显著降低;组织中T-AOC能力、GSH含量显著增加,MDA含量降低,均呈现剂量依赖性变化。病理学切片也显示石见穿总酚酸对肝保护的作用有剂量依赖关系。[结论]石见穿总酚酸对小鼠四氯化碳急性肝损伤具有一定的保护作用,作用机制可能与其抗氧化作用有关,石见穿总酚酸可增强组织抗氧化能力,降低四氯化碳引起的脂类过氧化,保护细胞膜免受损伤。

[Objective] To study the protective effect of phenolic acid from Salvia chinensis Benth. （PAS） against CCl4-induced acute liver injury in mice and its possible mechanism. [Method] Mice were divided into control group,model group randomly,Salvia chinensis Benth. was divided into 6 groups such as high,medium and low-dose treatment group and positive group （Silibinin）. Mice were pretreated by phenolic acid （i.g） for 5 days. Hepatic injury was induced by injecting 1 ml/kg 50% peanut oil solution of carbon tetrachloride,the contents of AST,ALT,T-AOC,MDA,GSH were determined after 24 h. [Result] Compared with model group,serum AST,ALT in PAS high,medium and low-dose groups reduced significantly; total antioxidant capacity （T-AOC）,reduced gluthione （GSH） increased,as well as MDA content decreased significantly,all above indexes changed in a dose-dependent way. Histopathological study showed that the protective effects of the PSA also indicated a dose-dependent manner. [Conclusion] The PAS has a protective effect on the carbon tetrachloride induced acute liver injury in mice,hepatoprotective effects of PAS may be related to its antioxidant characteristic. Total phenolic acids of Salvia chinensis Benth. can enhance anti-oxidant capacity of organizations,reduce carbon tetrachloride induced lipid peroxidation,and protect cell membranes from damage.