摘要
[目的]从分子水平上研究藏獒群体的遗传变异状况,为藏獒种质资源的保护提供依据。[方法]采用RAPD技术对不同地区的20份藏獒基因组DNA进行分析,并用群体遗传学软件PopGene32测定藏獒群体的有效等位基因数(Ne)、Nei氏平均预期基因杂合度(H)和Shannon遗传信息指数(I)。[结果]共扩增出369条带,其中339条表现出多态性,多态性位点百分率为91.86%。PopGene32软件得到的藏獒群体的Ne、H和I值分别为1.7512、0.4162和0.6021。[结论]藏獒群体内存在着丰富的遗传变异。
[Objective] To investigate the genetic variation of Tibetan mastiff at the molecular level and provide a basis for the protection of germplasm resources. [Methods] A total of 20 Tibetan mastiff blood samples in different regions were collected and the genomic DNA swere then analyzed using RAPD technique. The effective number of alleles （Ne）,Nei＇s average expected gene heterozygosity （H） and Shannon＇s information index （I） in Tibetan mastiff group were determined with population genetics software PopGene32. [Results] A total of 369 bands were amplified from all the blood samples,in which as many as 339 bands showed polymorphism. The percentage of polymorphic loci was 91.86%. The values of Ne,H and I were 1.751 2,0.416 2 and 0.602 1,respectively. [Conclusion] There is an abundance of genetic variations in the Tibetan mastiff group.
出处
《安徽农业科学》
CAS
北大核心
2010年第9期4954-4955,4966,共3页
Journal of Anhui Agricultural Sciences
基金
甘肃省科技攻关项目(GKC-97-27-5)
天水师范学院青年教师基金资助项目
关键词
藏獒
RAPD
遗传变异
Tibetan mastiff
RAPD
Genetic variation
