前列腺癌DU145细胞增殖和克隆形成与延伸因子1α基因表达的关系

Impact of elongation factor 1α expression change on proliferation and clone formation of prostate cancer cell line DU145

目的探讨延伸因子1a（EF-1α）基因表达与前列腺癌细胞株DU145细胞增殖、克隆形成等功能的关系。方法DU145细胞株分3组：对照组（未转染siRNA），转染对照组（转染随机siRNA）和实验组（转染EF-1α—siRNA）。应用RNA干扰技术特异性调低DU145细胞株EF-1α蛋白质水平，并通过蛋白质印迹法验证。应用体外细胞功能分析技术，比较EF-1α蛋白质水平调低后DU145细胞增殖、克隆形成能力的变化和差异。结果应用RNA干扰技术实现了特异性调低前列腺癌细胞株DU145中EF-1α的水平。转染随机siRNA不影响DU145细胞中EF-1α蛋白质水平。调低DU145细胞EF-1α蛋白质水平后，实验组DU145细胞第4～7天增殖率较对照组下降45．9％、53．5％、35．3oA和38．1％（P〈0．05）。实验组DU145细胞形成克隆数较对照组减少67．0％（P〈0．01）。结论调低EF-1α表达水平对前列腺癌细胞增殖、克隆形成等肿瘤相关生物学行为产生负面影响。Ep-1α基因在前列腺癌靶向治疗中可能成为适当的靶基因。

Objective To study the elongation factor 1α（EF-1α） gene functions in prostate cancer cell line DU145 in the aspects of cell proliferation and clone formation by using the RNA interfer- ence technique. Methods DU145 cell lines were divided into control group, transfection control group transfected with scramble siRNA and experimental group transfected with EF-1α siRNA. After transfecting EF-1α siRNA into DU145 cell line, the down-regulation of EF-1α expression in DU145 cell line was confirmed by Western blotting and immunofluorescence staining. Then, the cell proliferation and clone formation assays were carried on in these 3 groups of DU145 cells. Results Compared with controls, the specific down-regulation of EF-1α expression was achieved in experimental group only. Compared with control group, after the down-regualtion of EF-1α in DU145 cell line, the cell proliferation rate decreased from day 4 to day 7 after transfection by 45.9%, 53.5%, 35.3% and 38. 1%, respectively（P〈0.05）. The clone formation number in experimental group decreased by 67.0 （P〈0.01）. Conclusions The down-regulation of EF-1α has a negative impact on prostate cancer cell proliferation and clone formation. EF-1α might be an appropiate targeting gene in prostate cancer targeting therapy.