麻疯树籽蛋白提取工艺的研究

Extraction of Jatropha curcas seed protein

采用碱提酸沉法对麻疯树籽粕中的麻疯树籽蛋白进行了提取研究。通过单因素试验和正交试验得到的最优工艺条件为:碱提料液比1∶11,pH8.5,温度55℃,时间100 min;酸沉pH4.4,温度55℃,时间40 min。在该工艺条件下,麻疯树籽蛋白提取率达到76.71%,分离物蛋白纯度达到81.18%。

Alkaline extraction and acid precipitation were employed to produce protein from Jatropha curcas seed meal.The optimized conditions were obtained by orthogonal test as follows：alkaline extraction solid-liquid ratio 1∶11,pH 8.5,temperature 55℃,time 100 min;acid precipitation pH 4.4,temperature 55℃,time 40 min.Under the conditions,the extraction rate of Jatropha curcas seed protein was 76.71%,and the purity of protein reached 81.18%.