摘要
目的:建立测定人血浆中曲唑酮浓度的高效液相色谱法。方法:以DiamonsilTM C18反相柱(150mm×4.6mm,5μm)为色谱柱,流动相为0.03mol.L-1醋酸铵-甲醇(24∶76);流速:0.8mL·min-1;柱温:40℃;检测波长:255nm。以乙酸乙酯与二氯甲烷(80∶20)为提取剂。结果:曲唑酮的高、中、低(960.0,120.0,10.0ng·mL-1)3种浓度平均回收率分别为97.26%,98.42%,101.26%,日内、日间差RSD均低于7%(n=5);分析方法的检测限为5.0ng·mL-1;线性范围为10.0～960.0ng.mL-1。曲线方程:Y=0.36X+2.12,r=0.9992(n=9)。结论:该方法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究。
OBJECTIVE To establish a method for determining the concentration of trazodone in human plasma by HPLC. METHODS The drug from plasma was analyzed in a reverse phase HPLC system Ci8 column（ 150 mm × 4. 6 mm, 5μm） ; mobile phase consisted of 0. 03 mol. L a ammoniourn methanol（24： 76） ; the flow rate was 0. 8 mL.min^-1 ; the detection wavelength was at 255 nm Ethyl acetate and diehloromethane（80：21））was used as extracting solvent. RESULTS The average recoveries of trazodone in high, middle and low concentrations （960. 0,120. 0, 10. 0 ng·mL^-1） were 97. 26%, 98.42% and 101.26%, respectively. The intra-day and inter day variation（RSD）was less than 7%（71 = 5）. The calibration curve of trazodone showed good linearity,r= 0. 999 2（n = 9）,over the range of 10.0- 960.0ng.mL ：. The regression equation was Y = 0. 36X＋ 2. 12. r= 0. 999 2（n = 9）. The minimum detectable concentration of trazodone was 5. 0 ng·mL^-1. CONCLUSION The method is sensitive,accurate,easy and quick, which can be used for clinical drug monitoring and pharmacokinetic studies.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2010年第6期470-472,共3页
Chinese Journal of Hospital Pharmacy
基金
广东省自然科学基金资助项目(编号8151037001000001)
