青芥辣对离体肝吸虫囊蚴杀灭作用的研究

Killing Effects of Mustard on the Encysted Metacercaria of Clonorchis sinensis

目的研究青芥辣对离体肝吸虫囊蚴的杀灭作用。方法采用人工消化法从淡水鱼中获取囊蚴,分组(囊蚴20个/组),将其置于定量青芥辣软膏中,分别作用不同时间,显微镜下观察囊蚴的活动情况。结果青芥辣对肝吸虫囊蚴作用1～6min后,囊蚴内后尾蚴活动加强;作用7～180min后囊蚴活动力降低甚至开始死亡,死亡率范围为11.76%～100%;作用210min以后囊蚴全部死亡,死亡率达到100%。对照组囊蚴形态和后尾蚴活动无改变。结论青芥辣对肝吸虫囊蚴具有刺激和轻度杀灭作用,但在短时间内没有杀灭作用,所以期望用青芥辣杀灭肝吸虫囊蚴来达到预防肝吸虫感染的做法不可取。

Objective To evaluate the in vitro killing effects of mustard on the encysted metacercaria of Clonorchis sinensis. Methods Encysted metacercarias were collected from freshwater fishes by artificial digestion method. The encysted metacerearias were then divided into various groups with 20 metacercarias per group.The effect of mustard on their activities was then evaluated under a microscope. Results Enhanced activities were observed in the first 1 to 6 minutes after incubation of encysted metacercarias with musturd.Metacercaria became less active or even killed starting at 7 minutes （11.76%） and all metacercarias were killed at 210 minutes. Morphological alteration and activity of the encysted metacercarias were not changed in the control group. Conclusion Although mustard is cytotoxic to encysted metacercaria, it can not kill the metacercaria within a short period of time. It is not advisable to kill encysted metaeercaria by mustard for the purpose of preventing infection.