前S1抗原与丙氨酸氨基转移酶联合检测诊断乙型肝炎病毒复制

Clinical value of detection of pre-S1 antigen and alanine aminotransferase in patients with hepatitis B virus and the virus replication

目的:探讨前S1抗原与丙氨酸氨基转移酶(ALT)在诊断乙型肝炎病毒复制中的临床价值。方法:对150例乙型肝炎病毒s抗原(HBsAg)阳性的样本分别进行血清前S1抗原、乙型肝炎病毒-脱氧核糖核酸(HBV-DNA)和ALT的检测,前S1抗原采用酶联免疫吸附法,HBV-DNA采用实时荧光定量聚合酶链反应法,ALT采用全自动生化分析仪检测。结果:150例样本HBV-DNA均为阳性(≥103IU/ml),其中前S1抗原阳性116例,两者符合率为77.3%。前S1抗原阳性的116例中95例(81.9%)表现为乙型肝炎病毒的高复制(HBV-DNA>104IU/ml),同时51例ALT不升高的样本中35例(68.6%)HBV-DNA>104IU/ml。结论:前S1抗原是反映乙肝病毒复制及复制程度高低的指标,与血清ALT同时检测可以指导临床慢性乙型肝炎患者的定期复检及疗效判定。

Objective：To investigate the clinical significance of pre-S1 antigen （pre-S1 Ag）of hepatitis B virus （HBV）and alanine aminotransferase （ALT）detection on the diagnosis of virus replication.Methods：Pre-S1 Ag, HBV-DNA and ALT of 150 serum samples,which HBsAg were positive,were detected by ELISA,real-time fluorescence polymerase chain reaction assay and auto-biochemical analysis respectively.Results：One hundred and sixteen samples were pre-S1 Ag positive in all samples and were HBV-DNA positive（≥10^3IU/ml）,the coincident rate of pre-S1 Ag and HBV-DNA was 77.3%.Among the 116 pre-S1 Ag positive samples,95 of them （81.9%）had the higher level of HBV virus replication （HBV-DNA〉10^4IU/ml）.Fifty one samples had the common level of serum ALT,among them 35 samples were HBV-DNA〉10^4IU/ml.Conclusion：Pre-S1 Ag is a good indicator of HBV virus replication and the results which combined with serum ALT will guide the following up of chronic hepatitis B patients who need retest virus replication in a long time.