细粒棘球绦虫重组Bb-Eg95-EgA31疫苗诱导小鼠脾细胞亚群变化的研究

Changes of splenocyte subsets in mice immunized with recombinant Bb-Eg95-EgA31 vaccine against Echinococcus granulosus

目的探讨细粒棘球绦虫(Eg)重组双歧杆菌(Bb)-Eg95-EgA31疫苗免疫和Eg原头节攻击后小鼠的囊重抑制率及脾细胞亚群的变化。方法将细粒棘球绦虫重组Bb-Eg95-EgA31疫苗分别采用皮下注射、肌肉注射、鼻腔内接种和口服灌胃4种途径免疫BALB/c小鼠,免疫后8周每鼠用50个Eg原头节攻击感染,感染后25周剖杀小鼠,分离细粒棘球蚴包囊并称重,计算囊重抑制率;取脾,分离脾细胞,流式细胞仪检测脾CD4+和CD8+T细胞亚群的百分比,同时设有空载体、Bb和MRS对照。结果以上4种疫苗接种组小鼠的囊重抑制率分别为45.33%、41.33%、70.67%和62.67%;疫苗接种组的脾CD4+和CD8+T细胞亚群显著增加,鼻腔内接种和口服免疫组的的CD4+T细胞亚群和CD4+/CD8+比值显著高于皮下和肌肉注射组。结论CD4+和CD8+T细胞亚群在疫苗诱导的保护力中起重要作用。疫苗鼻腔内接种和口服免疫是两种较好的免疫途径。

To investigate the weight reduction of hydatid cyst and level of splenocyte subsets in mice immunized with recombinant Bifidobacteria bifidum（Bb） Bb-Eg95-EgA31 vaccine of Echinococcus granulosus（Eg） and challenged with Eg protoscoleces,BALB/c mice were immunized with the vaccine subcutaneously,intramuscularly,intranasally or orally.Then the mice were challenged with 50 Eg protoscoleces on 8th week after vaccination and killed on 25thweek after infection.The weight of hydatid cyst was measured and the reduction of the weight was calculated.The spleens were collected for splenocytes preparation.CD＋4 and CD＋8T cells were determined by flow cytometry（FCM）,and the blank vector,Bb or MRS were set as control.The hydatid cyst weight was reduced by 45.33%,41.33%,70.67% and 62.67% respectively for the 4 immunization groups.Number of CD＋4 and CD＋8 subsets in the immunization groups was more than that in the control group.CD＋4 and the ratio of CD＋4/CD＋8 in the intranasal or oral immunization group was much higher than that in the subcutaneous or intramuscular immunization group.It＇s concluded that CD＋4 and CD＋8T cells may play an important role in the protection induced by recombinant Eg95-EgA31 vaccine of Eg.In addition,intranasal and oral vaccinations could be the better ways for immunization.