感染曼氏裂头蚴小鼠的组织病理学观察

Histopathological observations on mice experimentally infected with plerocercoid

目的观察小鼠感染曼氏裂头蚴后的组织病理学改变,了解裂头蚴对组织的损害。方法从黑斑蛙体内检获裂头蚴,口服法以5条/只感染昆明小鼠,于感染后第2-10w分别处死小鼠进行组织病理学观察。结果（1）在皮下、肌肉、肝脏、肺腔及腮腺等处发现裂头蚴,主要为皮下肌肉组织;（2）组织病理观察：肉眼观察,寄生处的组织有弥漫性淤血斑点,囊包块形成;HE镜下观察,皮下组织结构破坏,点状的脂肪组织变性、坏死,伴纤维组织增生。肺组织肺泡壁毛细血管重度扩张充血,肺泡上皮中度增生,灶性肺组织坏死。肝组织少部分肝细胞轻度胞浆疏松化,小灶性肝细胞液化性坏死伴淋巴细胞浸润取代。腮腺组织结构破坏,重度变性、坏死,伴大量急慢性炎细胞浸润并脓肿形成。结论小鼠感染裂头蚴后,多组织器官有裂头蚴寄生,其中以皮下肌肉处多见。裂头蚴寄生可引起组织不同程度的增生、变性、坏死,组织结构破坏及大量炎症细胞浸润等病理变化。

To observe the histopathological changes of mice experimentally infected with plerocercoid,mice were infected orally by plerocercoids that were collected from Rana nigromaculatus（5 strips/mouse）,and were killed 2-10 weeks later to undergo the histopathological observation.Plerocercoids were found in the skin,muscle,liver,lung cavity of mice.There were diffuse congested spots and capsule formation;the fat tissue showed severe degeneration and necrosis.In the fibrous tissues,moderate proliferation of the enveloping adipose tissue was demonstrated.In addition,a large number of acute and chronic inflammatory cell infiltration and Abscess formation could be found.It is evident that the plerocercoids parasitize mainly in the subcutaneous tissues and muscles of mice.and mice experimentally infected with plerocercoids show different degree of histopathological changes.