骨骼肌成肌细胞移植对慢性心衰犬血液动力学的影响

Autologous skeletal myoblast transplantation improves hemodynamics dogs with chronic heart failure dogs

目的观察自体骨骼肌成肌细胞移植对慢性心力衰竭犬血液动力学的影响。方法19只慢性心衰犬模型建立后随机分为实验组(n=9):采用开胸直接心肌内注射自体骨骼肌成肌细胞;对照组(n=10):注射等量生理盐水。术前及术后5周行血液动力学检查,病理组织标本行Desmin和Brd-U免疫荧光双重染色。结果(1)术后5周,实验组dP/dtmax、MAP、LVSP明显升高(P<0.05),LVEDP明显下降(P<0.05),而对照组上述指标无显著性差异(P>0.05);(2)实验组心肌注射部位可见存活细胞,经Desmin和Brd-U免疫荧光双重染色证实来自于移植的骨骼肌成肌细胞。结论自体骨骼肌成肌细胞对慢性心力衰竭犬的血液动力学有明显改善作用。

Objective To test the effects of autologous skeletal myoblast transplantation on the hemodynamics in dogs with coronary microembolization-induced chronic heart failure （CHF）. Methods CHF models were successfully induced in 19 dogs and divided into ASMT group （n=9） and control group （n=10）. The myoblasts were injected into the embolized region in the 9 dogs of the ASMT group, and saline was injected in the control dogs, and the hemodynamics of the dogs were evaluated 5 weeks after the injections. Result Compared with saline injection, ASMT significantly increased dP/dtmax, MAP and LVSP （P0.05） and decreased LVEDP （P0.05） 5 weeks after myoblast transplantation. Desmin and Brd-U immunofluorescent staining showed myoblast survival at the injected sites in the dogs. Conclusion ASMT provides mild improvements in the hemodynamics of dogs with CHF.