补肾方剂对糖尿病大鼠牙周炎牙周组织形态结构及一氧化氮分布的影响

Effect of Tonifying Kidney Recipes on Periodontal Tissue and Nitric Oxide in Diabetes Rats

目的:观察补肾方剂对糖尿病大鼠牙周炎的治疗作用。方法:建立大鼠糖尿病牙周炎模型,补肾方剂灌服6周后,取标本,制作牙周组织切片,观察牙周组织形态结构的变化,测定牙槽骨高度丧失量和附着丧失量。测定牙龈组织中一氧化氮(NO)的含量。结果:糖尿病牙周炎中药治疗组的牙龈组织中NO的的含量最低。与正常组无显著性差异。牙槽骨高度丧失量和附着丧失量均明显低于糖尿病牙周炎组,有显著性差异。结论:补肾方剂促进糖尿病牙周炎牙周组织的修复。

Objective：To observe the effect of Tonifying Kidney Recipes（TKR）on Diabetes periodontitis in rats and to explore the mechanism of TKR for preventing the resorption of alveolar bone.Methods：To make the diabetes periodontitis model and to administer TKR at a dose of 3ml/3g/day in group TKR,at the 6th weeks the rats were killed respectively and the maxillary are collected.The gingiva of the left first maxillary molars was cut to measurate the amount of NO.The tissue sections of the right maxillary were stained for histological examination to observeperiodontium and caculate the loss of attachment and alveolar bonecrest height.Results：The amount of NO in group DP-TKR was the least.No great difference came up when comparing between the group normal and group DP-TKR;The loss of attachmentand alveolar bone crest height in group DP-TKR was remarkably less than that in group diabetes periodontitis.（P〈0.05）.Conclusion：TKR can play a role in promoting the restoration ofperiodontium and reduction the amount of NO.It can have effect on diabetes periodontitis in many ways.