复健片对脑梗死后大鼠脑组织神经生长导向因子的影响

Effect of Fujian Tablet on expression of axon guidance factor in rat after cerebral infarction

目的:观察复健片在不同时间点对脑梗死大鼠脑组织神经生长导向因子(slit)表达的影响,探讨其促进神经功能恢复的作用机制。方法:240只SD大鼠随机分为正常对照组、假手术组、模型组和药物组,并根据脑梗死病程每组又分为3d和1、2、4、6周共5个亚组。用改良的LongaEZ法制备大脑中动脉阻塞大鼠模型,药物组灌胃给予复健片水溶液,余各组分别灌胃给予同剂量的蒸馏水。用免疫组织化学法观察模型大鼠梗死周边区脑组织slit的表达。结果:模型组3d、1周、2周slit高于正常对照组;药物组slit在1,2,4,6周的表达显著高于同期模型组,于2周时达到高峰,后逐降低,6周时仍显著高于模型组,与模型组比较差异有非常显著性意义(P<0.01)。结论:slit在脑梗死后短时间内表达,复健片可增加slit的表达,有助于轴突生长和神经元胞体靶向迁移,促进神经功能恢复。

Objective： To explore the mechanism of Fujian Tablet in promoting neural regeneration by observing the effect of Fujian Tablet on the expression of slit in MCAO rats at different stages. Methods： 240 SD rats were randomly divided into normal control, sham operation, model and medicine groups, which were randomly divided into five subsets with 12 rats according to the day 3 and week 1, 2, 4, 6 stages. The rat models with middle cerebral artery occlusion were successfully established by the improved Longa EZ. The medicine group was gived with Fujian Tablet, other groups were given with commensurability distilled water. The expession of slit in the region around the infarction was observed by immunohistochemical method. Results： In the medicine group, slit -positive cells were obviously different with normal control group （P0.01）, increased and reached a peak on week 2, then decreased, but were obvious different with model group （P0.01） excluding the day 3 stage. Conclusion： The Fujian Tablet can increase the slit positive cells, and promote axon growth and neuron target migration.