VEGF-C、FLt4和结核菌L型感染在前列腺癌中的表达及临床意义

Expression and clinical significance of VEGF-C,FLt4 and tuberculosis infection L-type in prostate carcinoma

目的探讨血管内皮细胞生长因子-C（VEGF-C）与血管内皮细胞生长因子受体VEGFR-3（Flt4）,在前列腺癌中的表达以及结核菌L型感染率及临床意义。方法应用免疫组化和原位杂交和抗酸染色等方法检测65例前列腺癌（carcinoma of prostate,PCa）和30例良性前列腺增生（benign prostatic hyperplasia,BPH）中的VEGF-C、Fit4蛋白及mRNA的表达,以及结核菌L型的检出率。并对前列腺肿瘤主要临床资料和病理分级参数进行比较,用χ2检验进行统计学处理。结果VEGF-C、Flt4蛋白及mRNA阳性表达率和结核菌L型检出率,前列腺癌明显高于前列腺增生（P〈0.001-0.05）。VEGF-C、Flt4蛋白及mRNA阳性表达率和结核菌L型检出率与前列腺癌的临床分期、病理分级有明显差异（P〈0.05）。淋巴结转移组中VEGF-C、Flt4蛋白及mRNA的阳性表达率明显高于非转移组（P〈0.01）。结核菌L型检出率淋巴结转移组明显高于非转移组（P〈0.05）。结论VEGF-C、Flt4的蛋白及mRNA在前列腺肿瘤中不同程度异常表达以及结核菌L型检出率与肿瘤的临床分期、病理分级和转移呈正相关,提示这2种基因均可作为判断前列腺癌生物学行为及患者预后参考指标;结核菌L型感染可能有协同致瘤作用,与前列腺癌的发生发展可能有一定关系。因此研究VEGF-C、Flt4的阳性表达和结核菌L型感染与前列腺癌的关系,具有重要的临床应用价值。

Objective To explore the expressions of vascular endothelial growth factor-C（VEGF-C） and vascular endothelial growth factor receptor [VEGFR-3（Flt4）] in prostate carcinoma as well as the detection rate of L-type Mycobacterium tuberculosis infection and its clinical significance.Methods Immunohistochemistry,in situ hybridization and acid-fast stain were performed to 65 cases of prostate cancer（carcinoma of prostate,PCa） and 30 cases of benign prostatic hyperplasia（benign prostatic hyperplasia,BPH） for the expressions of VEGF-C,VEGFR-3（Flt4） and mRNA as well as the detection rate of TB L-type.The main clinical data for pathological classification parameters of prostate cancer were compared with χ^2 test for statistical analysis.Results The positive expressions of VEGF-C,Flt4 protein and mRNA as well as the L-TB detection rate of prostate cancer were significantly higher than those of benign prostatic hyperplasia（P0.001~0.05）.The positive expressions of VEGF-C,Flt4 protein and mRNA as well as the L-TB detection rate of prostate cancer at different clinical stage,histological grade were significantly different（P〈0.05）.The positive expressions of VEGF-C,Flt4 and mRNA as well as the L-TB detection rate of Lymph node metastasis group were significantly higher than those of non-metastasis group（P〈0.01 and P〈0.05 respectively）.Conclusion The abnormal expressions of VEGF-C,Flt4 and mRNA in prostate tumors as well as the detection rate of L-TB were positively correlated to clinical tumor stage,grade and metastasis,suggesting that both two genes can be used as the reference indicators for biological behavior of prostate cancer and prognosis of patients;L-TB infection may play synergistic tumorigenic role and be associated with the occurrence and development of prostate cancer.Researches on the relationship between prostate carcinoma and positive ex- pressions of VEGF-C and Flt4 as well as tubercular bacteria L-form infection can of important clinical significance.