摘要
目的探讨血管内皮细胞生长因子-C(VEGF-C)与血管内皮细胞生长因子受体VEGFR-3(Flt4),在前列腺癌中的表达以及结核菌L型感染率及临床意义。方法应用免疫组化和原位杂交和抗酸染色等方法检测65例前列腺癌(carcinoma of prostate,PCa)和30例良性前列腺增生(benign prostatic hyperplasia,BPH)中的VEGF-C、Fit4蛋白及mRNA的表达,以及结核菌L型的检出率。并对前列腺肿瘤主要临床资料和病理分级参数进行比较,用χ2检验进行统计学处理。结果VEGF-C、Flt4蛋白及mRNA阳性表达率和结核菌L型检出率,前列腺癌明显高于前列腺增生(P〈0.001-0.05)。VEGF-C、Flt4蛋白及mRNA阳性表达率和结核菌L型检出率与前列腺癌的临床分期、病理分级有明显差异(P〈0.05)。淋巴结转移组中VEGF-C、Flt4蛋白及mRNA的阳性表达率明显高于非转移组(P〈0.01)。结核菌L型检出率淋巴结转移组明显高于非转移组(P〈0.05)。结论VEGF-C、Flt4的蛋白及mRNA在前列腺肿瘤中不同程度异常表达以及结核菌L型检出率与肿瘤的临床分期、病理分级和转移呈正相关,提示这2种基因均可作为判断前列腺癌生物学行为及患者预后参考指标;结核菌L型感染可能有协同致瘤作用,与前列腺癌的发生发展可能有一定关系。因此研究VEGF-C、Flt4的阳性表达和结核菌L型感染与前列腺癌的关系,具有重要的临床应用价值。
Objective To explore the expressions of vascular endothelial growth factor-C(VEGF-C) and vascular endothelial growth factor receptor [VEGFR-3(Flt4)] in prostate carcinoma as well as the detection rate of L-type Mycobacterium tuberculosis infection and its clinical significance.Methods Immunohistochemistry,in situ hybridization and acid-fast stain were performed to 65 cases of prostate cancer(carcinoma of prostate,PCa) and 30 cases of benign prostatic hyperplasia(benign prostatic hyperplasia,BPH) for the expressions of VEGF-C,VEGFR-3(Flt4) and mRNA as well as the detection rate of TB L-type.The main clinical data for pathological classification parameters of prostate cancer were compared with χ^2 test for statistical analysis.Results The positive expressions of VEGF-C,Flt4 protein and mRNA as well as the L-TB detection rate of prostate cancer were significantly higher than those of benign prostatic hyperplasia(P0.001~0.05).The positive expressions of VEGF-C,Flt4 protein and mRNA as well as the L-TB detection rate of prostate cancer at different clinical stage,histological grade were significantly different(P〈0.05).The positive expressions of VEGF-C,Flt4 and mRNA as well as the L-TB detection rate of Lymph node metastasis group were significantly higher than those of non-metastasis group(P〈0.01 and P〈0.05 respectively).Conclusion The abnormal expressions of VEGF-C,Flt4 and mRNA in prostate tumors as well as the detection rate of L-TB were positively correlated to clinical tumor stage,grade and metastasis,suggesting that both two genes can be used as the reference indicators for biological behavior of prostate cancer and prognosis of patients;L-TB infection may play synergistic tumorigenic role and be associated with the occurrence and development of prostate cancer.Researches on the relationship between prostate carcinoma and positive ex- pressions of VEGF-C and Flt4 as well as tubercular bacteria L-form infection can of important clinical significance.
出处
《中国微生态学杂志》
CAS
CSCD
2010年第3期257-260,共4页
Chinese Journal of Microecology
基金
安徽省教育厅自然科学研究项目基金资助(KJ2007B141)