高效液相色谱法测定天麻素片中天麻素的研究

A method of HPLC to detemine concent of gastrodin in gastrodin tablet

目的：建立用HPLC法测定天麻素片中天麻素含量的方法。方法：样品经过处理后,用乙腈、磷酸水洗脱,用二级管阵列检测器的高效液相色谱仪测定。采用ODS C18分离;流动相为乙腈-水-磷酸（5＋95＋0.05）;流量1.00 ml/min;提取波长：220 nm。结果：该方法检出限为4.5 ng,相对标准偏差为4.54%,回收率为91.1%～96.6%。结论：该方法简便、快速、灵敏度高,有良好的稳定性和重现性,适用天麻素片中天麻素含量的测定。

Objective：To set up a method of HPLC to detemine the concent of gastrodin in gastrodin tablets.Methods：Samples after extraction were detected by HPLC.The sepration was carried out on a C18 column.Methanol-water-H3PO4（5＋95＋0.05） were used respectively as mobile phases in analyzing gastrodin.The flow rate was 1.0 ml/min.The decetive wavelength was set at 220 nm.Results：The RSD of this method was 4.54% for gastrodin.Detection limit of this method was 4.5ng.The recoveries range of this method was 91.1%~96.6%.Conclusion：This method is simple,rapid and accurate and can be used to determine gastrodin in gastrodin tablets.