高效离子色谱法测定氨基葡萄糖类化合物

Determination of glucosamine compounds by high-performance ion chromatography

目的：建立快捷、灵敏检测N-乙酰-D-氨基葡萄糖和D-氨基葡萄糖盐酸盐的离子色谱法。方法：采用离子色谱-积分脉冲安培检测法检测N-乙酰-D-氨基葡萄糖和D-氨基葡萄糖盐酸盐的含量,以NaOH淋洗液梯度淋洗,在CarboPac PA10高效阴离子交换柱（HPAEC）上实现分离,用Au工作电极、Ag/AgCl参比电极的脉冲安培检测器测定N-乙酰-D-氨基葡萄糖和D-氨基葡萄糖盐酸盐。结果：N-乙酰-D-氨基葡萄糖和D-氨基葡萄糖盐酸盐在0.02～20 mg/L范围内线性相关良好,相关系数r2分别为0.9999和0.9995。进样25μl、S/N=3时,方法检出限分别为0.005 mg/L和0.01 mg/L。精密性实验相对标准偏差为0.35%和0.76%;两种糖的加标回收率均在95.3%～101.6%之间。结论：建立的离子色谱-脉冲安培检测法检测N-乙酰-D-氨基葡萄糖和D-氨基葡萄糖盐酸盐,方法可靠、无需衍生、简便快捷、分离效果好、灵敏度高,可以在15 min内完成定量分析。

Objective：To establish a rapid and sensitive method for the analysis of N-acetyl-D-glucosamine and D-glucosamine hydrochloride using high proformance ion chromatography.Methods：The determination of N-acetyl-D-glucosamine and D-glucosamine hydrochloride was performed on an CarboPac PA10 colume with apulse amperometric detector with an Au working electrode and Ag/AgCl reference electrode.Results：The linear range was 0.02～20 mg/L,the correlation coefficient（r2） of N-acetyl-D-glucosamine and D-glucosamine hydrochloride was 0.9999 and 0.9995.Injection 25 μl,S/N=3,and the detection limit was 0.005 mg/L and 0.01 mg/L.The relative standard deviations was between 0.35% and 0.76%;two kinds of glucosamine compounds′ spiled recovery were between 95.3%~101.6%.Conclusion：The method of ion chromatography-integrated pulsed amperometric detection to detect N-acetyl-D-glucosamine and D-glucosamine hydrochloride is reliable,without derivatives,simple and quick,with good separation,high sensitivity and can be completed within 15 min of quantitative analysis.