2006～2008年东莞市副溶血性弧菌毒力因子分析

Analysis of virulence gene of Vibrio parahaemolyticus from 2006 to 2008 in Dongguan

目的：了解东莞地区2006～2008年副溶血性弧菌（Vibrio parahaemolyticus,Vp）分离菌株毒力因子的变化情况。方法：用PCR法及神奈川实验检测毒力因子。结果：食物中毒分离菌株tdh、trh基因检出率分别为85.2%（127/149）、8.7%（13/149）,外环境分离菌株tdh、trh基因检出率分别为6.3%（3/48）、2.1%（1/48）,所有菌株神奈川实验阳性率达91.9%（137/149）。结论：东莞地区副溶血性弧菌致病力较强,致病菌株大多携带tdh基因,对市民身体健康的潜在危害较大,应进一步加强对副溶血性弧菌的监测研究。

Objective：To investigate the virulence gene change of Vibrio parahaemolyticus from 2006 to 2008 in Dongguan.Methods：PCR method and Kanagaw phenomenon method was used to detect the virulence gene.Results：The positive rate of tdh and trh gene in the Vibrio parahaemolyticus strains from food poisoning samples was 85.2%（127/149） and 8.7%（13/149）,respectively.And it was 6.3%（3/48） and 2.1%（1/48） from the environment samples.The positive rate of Kanagaw phenomenon for all the Vibrio parahaemolyticus strains was 91.9%（137/149）.Conclusion：The pathogenicity of Vibrio parahaemolyticus strains in Dongguan is very strong,and most of them from food poisoning include tdh gene.It is necessary to strengthen the surveillance of Vibrio parahaemolyticus.