2008年温州市肠道病毒EV71型感染的检测与分析

Detection and analysis enterovirus 71 infection in general in Wenzhou city in 2008

目的:监测2008年温州市EV71感染情况,建立温州市手足口病EV 71型检测系统,预防和控制温州市EV71的大规模流行。方法:将样本中病毒进行核酸提取,通过荧光定量PCR、RT-PCR扩增及其电泳,分别检测肠道病毒通用型和EV71型、Cox A16;对EV71型病毒进行VP1测序和亚型分析。结果:检测284份标本的核酸样品,有179份样品为肠道通用病毒阳性,85份样品EV71阳性,4份样品CoxA16阳性。结论:温州市EV71感染主要集中在沿海区域,春夏季高发,易感人群为5岁以下儿童,主要流行的基因型为C4亚型。

Objective：Investigation on enterovirus 71（EV 71） infection in general in Wenzhou city in 2008,to establish EV 71 subtype of Hand,foot and mouth disease（HFMD） detection system,control and prevent large-scale prevalence of EV 71.Methods：To extract nucleic acid from virus as template of fluorescence quantitative PCR（FG-PCR） and RT-PCR,the amplification fragments were analyzed by DNA agrose gel electrophoresis.to detect enterovirus,EV71 and Cox A16,lastly,to sequence the PCR product of VP1 gene so confirm EV 71 subtype.Results：Among the 284 samples,179 samples were proved to be EV,85 samples to be EV71,4 samples to be CoxA16.Conclusion：The prevalence region of EV71 was coastland in Wenzhou City,the spring and summer of EV71 infection was the peak period,less than 5 years old children were susceptible for EV71,sequence analysis of vp1 gene of EV71 show the poluar subtype in wenzhou was C4 subtype.