摘要
为优化强化生物除磷系统胞内PHA、糖原以及多聚磷酸盐颗粒的测定方法,采用色谱与化学分析相结合的方法进行研究.其中,PHA的测定采用气相色谱法,糖原的测定采用稀盐酸消解加葡萄糖试剂盒法,多聚磷酸盐采用过硫酸钾氧化和钼锑抗光度法,经过方法优化得出4%酸化甲醇消解20h是提高PHA回收量的最佳条件;糖原在0.6mol/LHCl条件下消解5h提取量最大,临床中的葡萄糖氧化酶法试剂盒可以应用到本实验中;在120℃下氧化30min条件下多聚磷酸盐颗粒可以最大回收.经过加标回收实验等对这3个方法进行精密度、准确度分析,结果显示,在这3个条件下的3种方法都具有很高的精密度或准确度,而且简单易行,适合实验室及生产实践中的应用.
The combination of chromatogram and chemical analysis was adopted to optimize the methods selected to measure poly-P, glycogen and PHA intracellular polymer in EBPR system. PHA was measured by GC system, glycogen was degraded by dilute hydrochloric acid and analyzed by glucose commercial kit, and poly - P was oxidized by potassium persulfate and analyzed by molybdenum - antimony anti-spectrophotometric method. Testing of the proposed method for PHA analysis shows that 4% sulfuric acid concentration and degradation for 20 h are required for optimal PHB and PHV extraction, while 0. 6 mol/L HCl and digestion for 5 h produce the highest recovery of glycogen, and the medical glucose kit can be used in this research. Oxidation for 30 min at 120℃ is recommended for the recovery of poly - P. The results of precision analysis and recovery test with standard addition of these methods show that they possess high precision and are convenient for laboratory research and practical application.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2010年第2期207-211,共5页
Journal of Harbin Institute of Technology
基金
国家自然科学基金海外青年学者合作项目(50628808)
城市水资源与水环境国家重点实验室开放研究项目(QAK200802)
北京市教委科技创新平台项目(PXM2008-014204-050843)
关键词
强化生物除磷
聚磷
多聚脂肪酸
糖原
聚磷菌
enhanced biological phosphorus removal system
poly -phosphate
polyhydroxyalkanoates
glycogen
phosphorous accumulating organisms