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乳源酵母Pichia fermentans的抗氧化特性 被引量：3

Antioxidant activity of Pichia fermentans isolated from raw milk
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摘要 为得到新的天然抗氧化剂,采用DPPH法和抑制脂质过氧化法对11株来源于牛乳的酵母菌抗氧化活性进行测定,包括他们的完整细胞和无细胞提取物,发现Pichia fermentans BY5有较好的抗氧化活性.分析不同条件下P.fermentans BY5细胞提取物的抗氧化活性,发现培养基的起始pH值、培养时间、培养基种类对酵母菌的抗氧化活性和蛋白质含量影响显著(P<0.05);而抗氧化活性与蛋白质含量显著相关(P<0.01).将酵母BY5在pH6的麦芽培养基中培养2d后清除DPPH活性可达60%;在pH6的YPD培养基中培养4d后抑制脂质过氧化能力达到74%. To obtain new natural antioxidant, 11 yeast strains isolated from raw milk were screened by determining their capability to scavenge 1,1 - diphenyl - 2 picrylhydrazl （ DPPH ） free radical and inhibit linoleic acid peroxidation, including their intact cell and cell-free extracts. It was found that Pichia fermentans BY5 showed excellent antioxidant activity. Then its antioxidant activity under different initial pH and incubation time as well as culture media of wort, YPD and PDA was analyzed. The results revealed that all these conditions significantly affected the antioxidant activity （ P 〈 0. 05 ）, and the protein contents showed a remarkable correlation with antioxidant capability （ P 〈0. 01 ）. In addition, the capability of scavenging DPPH of BY5 extraction reached 60% when being cultured with initial pH 6 in wort for 2 d, and the inhibition of linoleic acid peroxidation reached 74% in YPD for 4 d.
作者 陈历水 马莺 Jean-Louis MAUBOIS 刘巧红
机构地区 哈尔滨工业大学食品科学与工程学院 法国农业科学研究院雷恩乳品中心
出处 《哈尔滨工业大学学报》 EI CAS CSCD 北大核心 2010年第2期292-296,共5页 Journal of Harbin Institute of Technology
关键词 PICHIA fermentans 抗氧化活性 蛋白质 原料乳 Pichia fermentans antioxidant activity protein raw milk
分类号 TS252.1 [轻工技术与工程—农产品加工及贮藏工程]
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参考文献10

  • 1NAYLIN N, TAING O, HASHINAGA F, et al. Antioxidant activity of sugar - tolerant yeast Zygosaccharomyces rouxii [ J ]. Food Bioteeh, 2005, 19 : 107 - 120.
  • 2RASHID M H, KATO F, MURATA A. Effect of microorganisms on the production of lipid and fatty acid composition of fermented fish meal [ J ]. Biosci Biotech Biochem, 1992,56 : 1058 - 1062.
  • 3YEN G C, LEE C A. Antioxidant activity of extracts from molds [J]. J Food Prot, 1996, 59:1327 -1330.
  • 4FREITAS de I, PINON N, BERDAGUE J L, et al. Kluyveromyces lactis but not Pichiafermentans used as adjunct culture modifies the olfactory profiles of Cantalet cheese [J]. J Dairy Sci, 2008, 91:531 -543.
  • 5KULLISAAR T, SONGISEPP E, MIKELSAAR M, et al. Antioxidative probiotic fermented goats ' milk decreases oxidative stress - mediated atherogenicity in human subjects [J]. Br J Nutr, 2003, 90:449-456.
  • 6LOWRY O H, ROSEBROUGH N J, LEWIS F A, et al. Protein measurements with the folin phenol reagent [J]. J Biol Chem, 1951, 193:265 -275.
  • 7LIN M Y, CHANG F J. Antioxidative effect of intestinal bacteria Bifidobacterium longum TCC 15708 and Lactobacillus acidophilus ATCC 4356 [ J ]. Dig Dis Sci, 2000, 45 : 1617 - 1622.
  • 8JEHRIG S C, ROHN S, KROH L W, et al. Antioxidative activity of ( 1 - 3 ), ( 1 - 6) - β - D - glucan from Saccharomyces cerevisiae grown on different media [J]. LWT, 2008, 41:868-877.
  • 9JEHRIG S C, ROHN S, KROH L W, et al. In vitro potential antioxidant activity of ( 1 - 3 ), ( 1 - 6) - D - Glucan and protein fractions from Saccharomyces cerevisiae cell walls [ J ]. J Agric Food Chem, 2007, 55:4710 - 4716.
  • 10NAYLIN N, SUGANUMA T, TAING O. Antioxidant activities of compounds isolated from fermented broth of Zygosaccharomyces rouxii [ J ]. Food Biotech, 2006, 20:131-141.

同被引文献50

  • 1FLEET G H. A Review: Yeasts in dairy products [ J ]. Journal of Applied Bacteriology, 1990, 68:199-211.
  • 2ROHM H, ELISKASES- LECHNER F, BRUER M. Diversity of yeasts in selected dairy products [ J ]. Journal of Applied Bacteriology, 1992, 72 : 370 - 376.
  • 3DEAK T, BEUCHAT L R, GUERZONI M E, et al. A collaborative study on media for the enumeration of yeasts in foods [ J ]. International Journal of Food Microbiology, 1998, 43 : 91 -95.
  • 4ROOSTITA R, FLEET G H. Growth of yeasts in milk and associated changes to milk composition [ J ]. Inter- national Journal of Food Microbiology, 1996, 31 : 205 - 219.
  • 5PSOMAS E I, FLETOURIS D J, LITOPOULOU- TZANETAKI E, et al. Assimilation of cholesterol by yeast strains isolated from infant feces and feta cheese [ J ]. Journal of Dairy Science, 2003, 86: 3416 - 3422.
  • 6KUMURA H, TANOUE Y, TSUKAHARA M, et al. Screening of dairy yeast strains for probiotic applica- tions [ J]. Journal of Dairy Science, 2004, 87:4050 - 4056.
  • 7ELMER H M, JAMES L S. Applied Dairy Microbiology [ M ]. New York : Marcel Dekker, 2001.
  • 8DE FREITAS I, PINON N, MAUBOIS J L, et al. The addition of a cocktail of yeast species to Cantalet cheese changes bacterial survival and enhances aroma compound formation [ J ]. International Journal of Food Microbiology, 2009, 129 : 37 - 42.
  • 9GORI K, MORTENSEN H D, ARNEBORG N, et al. Ammonia production and its possible role as a mediator of communication for Debaryomyces hansenii and other cheese-relevant yeast species [ J ]. Journal of Dairy Science, 2007, 90:5032-5041.
  • 10WANG S Y, CHEN H C, LIU J R, et al. Identification of yeasts and evaluation of their distribution in Taiwan Residents kefir and viili starters[ J ]. Journal of Dairy Science, 2008,91 : 3798 - 3805.

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哈尔滨工业大学学报
2010年 第2期

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