山茱萸多糖对雄性大鼠性功能损伤的影响

Effect of Fructus Corni polysaccharides on damaged sexual function of male rats

目的:观察山茱萸多糖(Fructus Corni polysaccharides,FCP)对半去势大鼠性功能的影响。方法:将70只SD雄性大鼠随机分为正常对照组(灌胃生理盐水),阴性对照组(摘除右侧睾丸+灌胃生理盐水),阳性对照组(摘除右侧睾丸+丙酸睾丸素,皮下注射2 mg.kg-1.d-1),FCP实验组(摘除右侧睾丸+灌胃FCP 10,50,100,150 mg.kg-1.d-1),通过交配试验及阴茎勃起试验观察FCP对半去势大鼠性功能的影响,以双抗放射性免疫法(RIA)测定血清性激素水平。结果:正常对照组、阳性对照组、FCP各实验组能明显缩短阴茎勃起潜伏期、爬高潜伏期,提高爬高动物百分率;提高血清T水平,降低E2水平;提高其包皮腺、精囊腺-前列腺、提肛肌等脏器系数、精子计数及活力,与阴性对照组相比差异有显著性(P<0.01,P<0.05)。结论:FCP可以改善半去势大鼠的性功能,其机制可能是通过促进与改善下丘脑-垂体-性腺轴的功能调节系统而起作用。

Objective ： To observe the effects of Fructus Corui polysaccharides （FCP） on sexual function of hemicastrated rats. Method ：70 male SD rats are randomly divided into 7 groups with their right testis extirpated except for normal control group. Normal control group and negative control group are given saline （ig） while positive control group are injected hypodermically testosterone propionate at dose of 2 mg·kg^-1·d^-1. FCP control groups are given FCP separately at dose of lO, 50, 100, 150 mg·kg^-1·d^-1 （ig） . Mating test and erective test are observed. The levels of serum sex hormone T, LSH, FSH, E2 are detected with the Radioimmunoassay （RIA）. Result： Incubation period of penis erection and mounting are shortened in FCP control groups and positive control group, and the percentage of mounting rats is increased. The level of serum sex hormone T is increased, but estradiol level is reduced. The organ coefficient of foreskin gland and seminal vesicle-prostate gland as well as sperm count and vigor are increased significantly （P 〈 0. 01 or P 〈 0. 05 ）. Conclusion： FCP can increase the sexual function of hemicastrated rats. The mechanism is probably through adjustment of the hypothalamus -pituitary-gonadal axis.