不同临床类型慢性乙型肝炎HBsAg和HBV DNA水平比较

Comparison of Level of HBsAg and HBV DNA in Different Clinical Types of Chronic Hepatitis B

目的探讨不同临床类型慢性乙型肝炎(慢性乙肝)对HBsAg和HBV DNA水平的影响。方法分别采用酶联免疫吸附试验(ELISA)和聚合酶链式反应(PCR)技术检测91例慢性乙肝患者的HBsAg和HBV DNA,并对HBV DNA的检测结果进行对数转换;对不同临床类型测定结果进行统计分析。结果不同临床类型慢性乙型肝炎HBsAg、HBV DNA水平差异均有统计学意义(P<0.05),其中慢重肝组与肝硬化组HBsAg和HBV DNA水平比较差异无统计学意义,P值及95%可信区间分别为0.334、-85.05～29.20和0.510、0.59～1.17;慢重肝组HBsAg水平低于慢乙肝组,HBV DNA水平高于慢乙肝组,P值及95%可信区间分别为0.000、163.21～-56.40和0.020、-1.81～-0.16;肝硬化组HbsAg水平低于慢乙肝组,HBV DNA水平高于慢乙肝组,P值及95%可信区间分别为0.006、-139.76～-24.00和-0.005、-2.17～-0.39。结论从HBsAg和HBV DNA水平看,HBsAg和HBV DNA的水平在慢乙肝组的均值显著高于肝硬化组,而肝硬化组又高于慢重肝组;提示慢性乙肝不同临床类型对HBsAg和HBV DNA水平产生一定的影响。

Objective To Comparison of level of HBsAg and HBV DNA in different clinical types of chronic hepatitis B.Methods Peripheral blood was collected from 91 patients with chronic hepatitis B.The HBsAg and HBV DNA in the sera of patients with chronic hepatitis B were tested by ELISA and PCR techniques respectively.The index numbers of HBV DNA were changed into constants by logarithm.Results The level of HBsAg and HBV DNA in different clinical types of chronic hepatitis B showed significant difference（P〈0.05）,The significance and the 95% confidence interval were 0.334,-85.05 to 29.20 and 0.510,0.59 to 1.17,respectively,with no significant difference in comparison of HBsAg and HBV DNA level between the severe hepatitis B and the cirrhosis groups.There was significant difference in the comparison of HBsAg and HBV DNA level between the severe hepatitis and chronic hepatitis B groups,and the significance and the 95% confidence interval were 0.000,163.21to -56.40,and 0.020,-1.81 to -0.16,respectively.There was significant difference in comparison of HBsAg and HBV DNA level between the cirrhosis and chronic hepatitis B groups,and the significance and the 95% confidence interval were 0.006,-139.76 to -24.00 and 0.005,-2.17 to -0.39,respectively.Conclusion The mean difference of HBsAg and HBV DNA level in chronic hepatitis B group is higher than those of cirrhosis group,the mean difference of HBsAg and HBV DNA level in cirrhosis group is higher than those of severe hepatitis B.The clinical types of chronic hepatitis B might affect the HBsAg and HBV DNA level.