胃旁路术对非肥胖2型糖尿病GK大鼠的降糖作用

The antidiabetic effect of gastric bypass surgery in a non-obese type 2 diabetes rat model

目的:观察胃旁路术(gastric bypass,GBP)对非肥胖型2型糖尿病GK大鼠(Goto-Kakizaki rats)的降糖作用及其机制。方法:雄性GK大鼠和SD大鼠各20只,随机分为GK手术组、GK假手术组、SD手术组、SD假手术组,每组10只。测定术前及术后第1、2、4、8周各组体重、空腹血糖(FPG)、糖耐量(OGTT)、血浆胰岛素(INS)、血浆胰高血糖素样肽-1(GLP-1)水平。结果:术后8周,GK手术组FPG和OGTT由术前的(5.0500±0.395)mmol/L和(22.7900±3.525)mmol/L下降到(4.0125±0.476)mmol/L和(12.4875±1.173)mmol/L,血浆INS和GLP-1由术前的(674.00±224.372)pg/mL和(6915.00±1566.860)pg/mL上升到(873.63±115.920)pg/mL和(8508.75±1247.013)pg/mL,差异均有显著性(P<0.05)。结论:GBP能显著改善非肥胖型2型糖尿病GK大鼠糖代谢,与术后肠胰岛轴改变血浆INS、GLP-1水平升高有关。

Objective To investigate the antidiabetic effect and potent mechanism of gastric bypass （GBP） surgery in non-obese type 2 diabetes Goto-Kakizaki （GK） rats. Methods Male rats （20 GK mice and 20 SD mice） were randomized into 4 groups： group l, GK operation, group 2, GK sham operation, group 3, SD operation and group4, SD sham operation. Body weight, fasting plasma glucose, oral glucose tolerance （OGTF）, plasma insulin level and glucagonlike peptide-1 level（GLP-1 ） were determined before surgery and 1, 2, 4 and 8 weeks after surgery. Results The fasting plasma glucose level decreased from （5.050 0 ± 0.395）mmol/L before surgery to （4.012 5 ± 0.476）retool/L, and the OGTF decreased from （22.790 0 ± 3.525）mmol/L before surgery to （ 12.487 5 ± 1.173） mmol/L 8 weeks after surgery in GK rats. While the plasma insulin level increased from （674.00 ± 224.372）pg/mL before surgery to（873,63 ± 115.920）pg/mL, and the plasma GLP-1 level increased from （6 915.00 ± 1 566.860）pg/mL before surgery to（8 508.75 ± 1 247.013）pg/mL 8 weeks after surgery in GK rats （P 〈 0.05）. Conclusions GBP can significantly improve glycometabolism in non-obese type 2 diabetes rat models, and the hypoglycemic effects are associated with the increase of plasma insulin and CLP-1 following the change of enteroinsular axis post operations.