cDNA微阵列分析穿刺手术所致BALB/c小鼠肝损伤基因表达谱变化

Gene Expression Profile Change of Hepatic Injury in BALB/c Mouse Caused by cDNA Microarray Puncture Operation

目的分析肝穿刺术后BALB/c小鼠肝脏基因表达谱的变化。方法10只BALB/c随机分为两组;实验组和正常组,每组各5只;实验组小鼠开腹直视下肝左叶注生理盐水,8天后采集肝组织标本并提取RNA,cDNA微阵列分析25 000个基因在实验组和正常组小鼠肝组织中的表达水平差异。结果肝穿刺术后共有82条差异基因,其中52条基因表达上调,30条基因表达下调;功能聚类可分为代谢、转录调节、转运、信号转导、炎症反应、细胞增殖与凋亡、细胞周期与细胞骨架及细胞粘附等8类。结论肝穿刺术所致小鼠肝脏基因表达谱发生的改变,涉及多个基因表达调控途径。

Objective To analyze mRNA expression profile of the liver in BALB/c mouse after liver puncture.Methods Ten BLAB/c mice were randomly divided into two groups： experimental group（5 mice） and normal group（5 mice）.Liver puncture was performed by intra-hepatic injection of saline under a surgery microscope method.After 8 days,the mouse liver samples were collected and used to extract total RNA.The expression levels of approximately 25 000 genes were analyzed in the BALB/c mouse liver injected with saline comparing with the liver from normal group by cDNA microarray.Results The microarray showed that the expression levels of 82 genes were remarkably altered,52 of the genes were up-regulated and 30 genes were down-regulated.The altered genes were associated with metabolism,regulation of transcription,transport,signal transduction,inflammatory response,cell proliferation and apoptosis,cell cycle and cytoskeleton,cell adhesion and other processes.Conclusions Many differentially expressed genes with different functions interact with each other to resist liver injury by puncture.