关龙胆乙醇提取物在急性肺损伤中的作用研究

Protective Effects of Ethanol Extract of Radix et Rhizoma Gentianae on Acute Lung Injury

目的:研究关龙胆乙醇提取物对急性肺损伤的保护作用。方法:NIH雄性小鼠48只,随机均分为空白(等量生理盐水)、模型(等量生理盐水)、醋酸地塞米松(0.005g·kg-1)及关龙胆乙醇提取物高、中、低剂量(10、5、2.5g·kg-1)组。灌胃给药,每天1次,连续5d。末次灌胃后以脂多糖(LPS,2.5mg·kg-1)气管滴注复制急性肺损伤模型。复制模型24h后以酶联免疫法检测血浆前列腺素E2(PGE2)、白介素-10(IL-10)和环氧化酶-2(COX-2)的含量。结果:与模型组比较,关龙胆乙醇提取物高、中、低剂量组血浆PGE2和COX-2水平显著降低(P<0.05或P<0.01),血浆IL-10水平显著升高(P<0.05或P<0.01)。结论:关龙胆乙醇提取物可能通过抑制COX-2表达、降低PGE2生成,并促进IL-10的生成对急性肺损伤产生保护作用。

OBJECTIVE： To investigate the protective effects of the ethanol extract of Radix et Rhizoma Gentianae on acute lung injury （ALI）. METHODS： 48 NIH male mice were randomized into blank group （normal saline）, model group （normal saline）, dexamethasone acetate group （0.005 g.kg^-1）, ethanol extract of Radix et Rhizoma Gentianae groups （high-dosage of 10 g. kg^-1, medium dosage of 5 g. kg^-1 and low dosage of 2.5 g. kg^-1）. The groups were administered via i.g gtt once a day for five days. ALl model was induced by intratracheal injection of lipopolysaccharide （LPS, 2.5 mg. kg^-1） after last treatment. ELISA method was applied to determine the content of prostaglandin E2 （PGE2）, interleukin-10 （IL-10） and cycloxygenase-2（COX-2） at 24 h after modeling. RESULTS： As compared with model group, the content of PGE2 and COX-2 in ethanol extract of Radix et Rhizoma Gentianae groups were decreased significantly （P〈0.05 or P〈0.01） while the content of IL-10 was increased significantly （P〈0.05 or P〈0.01）. CONCLUSION： Ethanol extract of Radix et Rhizoma Gentianae protect lung by inhibiting the expression of COX-2, reducing the production of PGE2 and promoting the generation of IL- 10.