HPLC法同时测定黄芩中5种黄酮类成分的含量

Determination of 5 Kinds of Flavonoids in Scutellaria baicalensis by HPLC

目的:建立以高效液相色谱法同时测定黄芩中5种黄酮类成分含量的方法,并采用该方法分析天津市15家零售药店中黄芩的质量。方法:色谱柱为AgilentZorbaxSB-C1(8150mm×4.6mm,5μm),流动相为含0.1%甲酸的乙腈-水溶液(梯度洗脱),检测波长为278nm。结果:黄芩苷、汉黄芩苷、黄芩素、汉黄芩素和千层纸素A的进样量分别在0.085～2.727μg(r=0.9999)、0.021～0.672μg(r=0.9999)、0.037～1.181μg(r=0.9999)、0.016～0.504μg(r=0.9993)、0.006～0.185μg(r=0.9997)范围内与各自峰面积积分值呈良好的线性关系;平均回收率分别为102.5%(RSD=2.63%)、97.09%(RSD=2.49%)、102.1%(RSD=2.87%)、97.5%(RSD=2.41%)和100.4%(RSD=2.86%)(n均为6)。结论:本方法简便、准确、重复性好,可用于黄芩药材的质量控制。

OBJECTIVE ： To establish an HPLC method for the determination of the contents of 5 kinds of flavonoids in Scutellaria baicalensis and quality analysis of these from 15 retail drug stores in Tianjin. METHODS： The separation was performed on Agilent Zorbax SB-C18 column （150 mm × 4.6 mm, 5 um） with mobile phase consisted of 0.1% formic acid acetonitrile-water （gradient elution）. The detection wavelength was set at 278 nm. RESULTS： The linear range were 0.085-2.727ug for baicalin （r=0.999 9）, 0.021--0.672 ug for wogonoside（r=0.999 9）, 0.037-1.181ug for baicalein（r=0.999 9）, 0.016-0.504 ug for wogonin （r=0.999 3） and 0.006-0.185 ug for oroxylin A（r=0.999 7）. The average recoveries were 102.5% （RSD=2.63%）, 97.09% （RSD=2.49%）, 102.l% （RSD=2.87%）, 97.5% （RSD=2.41%） and 100.4% （RSD=2.86%）, respectively（n=6）. CONCLUSION： This method is simple, accurate and reproducible for the quality control ofS. baicalensis.