应用导流杂交基因芯片技术检测人乳头状瘤病毒亚型的临床意义

Clinical Significance of HPV Genotypes Detection by using Flow Through Hybridization and Gene Chip

[目的]探讨人乳头状瘤病毒（HPV）不同型别的感染情况及其分型检测的临床意义。[方法]用导流杂交基因芯片技术对523例门诊首诊人群的宫颈脱落细胞标本进行HPV基因诊断和分型。[结果]①523例标本中HPV整体感染率为36.1%（189/523）,单重感染率为31.4%（164/523）;复合型别感染率为4.8%（25/523）,包括2.9%（15/523）的双重感染及1.9%（10/523）的多重感染。②20-29岁和50-59岁组的感染率较高。③HPV阳性人群其宫颈高度病变的发生率（45.0%,85/189）显著高于HPV阴性人群（9.6%,32/334）。[结论]HPV分型检测对筛选宫颈高度病变有重要意义。导流杂交基因芯片技术适合临床筛查HPV感染及基因分型。

[Objective] To discuss the distribution of human papillomavirus（HPV） subtypes and the clinic significance.[Methods] Totally 523 cervix cellular specimen were analyzed by flow through hybridization and gene chip（HybrMax） to detect the genetic subtypes of HPV.[Results] ①General infection rate of HPV was 36.1%（189/523）,single subtype infection rate was 31.4%（164/523）,and complex subtypes infection rate was 4.8%（25/523）,which involved 2.9%（15/523） dual-subtype infection and 1.9%（10/523） multi-subtype infection.②The rates for age groups 20-29 and 50-59 were higher than the others.③The prevalence of high cervical lesion（45.0%,85/189） in positive population was significantly higher than that in negative population（9.6%,32/334）.[Conclusion] The detection of HPV subtypes is significant to screen the potential cervical lesion and HybrMax technology is suitable for clinical screening of HPV infection and gene typing.