降脂壮骨中药对食饵性高脂血症大鼠骨组织骨形态发生蛋白2、雌激素受体表达的影响

Influence of Chinese medicine of Jiangzhi Zhuanggu on bone morphogenetic protein and estrogen receptor expression in bone tissue of hyperlipidemia-induced osteoporosis rats

背景:研究表明活性骨形态发生蛋白2水平的降低可导致严重的类骨质疏松症状,并发现成骨细胞、破骨细胞及骨髓基质细胞上有雌激素受体的存在,认识到雌激素对骨组织具有直接作用。目的:观察降脂壮骨中药对食饵性高脂血症诱发骨质疏松大鼠骨组织骨形态发生蛋白2、雌激素受体表达的影响。方法:健康SD大鼠27只,随机分为3组:正常对照组:上午和下午灌胃生理盐水5mL/kg;高脂血症诱发骨质疏松模型组:上午灌胃高脂饮食5mL/kg,下午灌胃生理盐水5mL/kg;降脂壮骨中药组:上午灌胃高脂饮食5mL/kg,下午灌胃降脂壮骨中药水提液5mL/kg。持续干预8周后采用免疫组织化学方法,对其骨组织中骨形态发生蛋白2、雌激素受体的表达水平进行检测,应用原位杂交方法对骨组织中雌激素受体的mRNA水平进行检测。结果与结论:与模型组比较,降脂壮骨中药组骨组织中骨形态发生蛋白2、雌激素受体表达显著增强(P<0.01),雌激素受体的mRNA表达水平增高。证实降脂壮骨中药能够使食饵性高脂血症诱发骨质疏松大鼠成骨细胞骨形态发生蛋白2、雌激素受体的表达水平增高,起到改善骨质疏松的作用。

BACKGROUND： Active bone morphogenetic protein （BMP-2） reduction can cause severe symptoms like osteoporosis. In addition, there is estrogen receptor （ER） in osteoblast, osteoclast and bone marrow stromal cells, indicating a direct effect of estrogen on bone tissues. OBJECTIVE： To investigate the effect of Chinese medicine of Jiangzhi Zhuanggu on BMP-2 and ER expression in rats with prey hyperlipidemia induced osteoporosis. METHODS： A total of 27 adult SD rats, of either gender, weighing 180-230 g, were randomly divided into three groups. In the normal control group, rats were intragastrically infused with 5 mL/kg normal saline every morning and afternoon. In the model group, the rats were infused with 5 mL/kg high-fat diet in the morning and 5 mL/kg normal saline in the afternoon. In the Chinese medicine group, 5 mL/kg high-fat diet was infused in the morning and 5 mL/kg Chinese medicine of Jiangzhi Zhuanggu water extract in the afternoon. After 8 weeks, expression levels of BMP-2 and ER in bone tissue was detected with immunohistological methods, and ER mRNA level of bone tissue in rats was detected by in situ hybridization. RESULTS AND CONCLUSION： Compared with the model group, the BMP-2 and ER expression in the bone tissue was significantly increased （P 0.01）, and ER mRNA level increased following Chinese medicine treatment. Results show that Chinese medicine of Jiangzhi Zhuanggu could increase BMP-2 and ER expression in the osteoporosis bone tissue, and improve osteoporosis effectively.