摘要
目的研究夏枯草对淋巴瘤细胞生长的影响及其可能的机制。方法参考临床常用剂量，采用50g/L夏枯草（夏枯草组）、50g/L夏枯草及20umot／L．c—Jun氨基末端激酶（JNK）特异抑制剂SP600125（SP600125组）处理Raji细胞，以加入同体积生理盐水处理的Raji细胞作对照，应用噻唑蓝比色法（M丌法）检测各处理方式下细胞增殖率，通过Western印迹检测JNK、c—Jun磷酸化水平和半胱氨酰天门冬氨酸（Caspase-3）的表达，流式细胞术（FCM）检测细胞凋亡率。结果夏枯草细胞增殖率（67．32±1．96）％低于其余各组（P〈0．05）；JNK磷酸化水平（0．48±0．03）和c-Jun磷酸化水平（0．46±0．04）在夏枯草组均明显增高（P〈0．05），SP600125可抑制c—Jun磷酸化（0．43±0．01）；夏枯草组的Caspase-3表达（1．35±0．07）高于其他各组，SP600125使其表达减少（0．79±0．06，P〈0.05）；细胞凋亡率在夏枯草组中最高（25．32±5．27）％（P〈0．05）。结论夏枯草可以明显抑制Raji细胞的生长，这种抑制作用可能是通过激活JNK信号转导通路和Caspase途径导致细胞凋亡实现的。
Objective To characterize the effect of Prunella vulgaris upon lymphoma cells and its possible mechanisms. Methods Raji cells were subjected to different therapeutic schemes： Prunella vulgaris （50 g/L） group, P. vulgaris （50 g/L） ＋ SP600125 （20 umol/L, JNK inhibitor） （SP600125 group） ; Raji cells in physiological saline served as the control. MTF assay was used to measure the cellular proliferation. Phosphorylation of JNK, c-Jun and the expressions of Caspase-3 were determined by Western blot. Cell apoptosis was analyzed by FCM. Results The prnliferation rate of cells in the P. vulgaris group （67.32± 1.96） % was significantly lower than that in the other groups （ P 〈 0. 05 ）. The phosphorylation of JNK （0. 48 ± 0. 03 ） and the phosphorylation of c-Jun （0.46± 0. 04） significantly increased in the P. vulgaris group （P 〈 0. 05 ） and SP600125 inhibited the phosphorylation of c-Jun （0. 43 ± 0. 01 ）. The expression of Caspase-3 in the P. vulgaris group （ 1.35 ± 0. 07） was higher than that in the other groups. And such an effect could be blocked by SP600125 （0.79 ＋-0.06） （P 〈0.05）. The rate of cellular apoptosis was the highest in the P. vulgaris group （25.32 ± 5.27 ） % （ P 〈 0. 05 ）. Conclusion P. vulgaris shows a strong inhibitory effect upon the growth of lymphoma cell line Raji probably through the activation of JNK pathway and caspase channel and then apoptosis.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2010年第10期690-693,共4页
National Medical Journal of China
基金
国家自然科学基金（30972457）
